DR6 Active Protein | TNFRSF21 active protein

DR6 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122646

• Gene Names: Tnfrsf21; DR6; TR7; R74815; AA959878
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DR6; DR6 Protein; Mouse; Recombinant (His Tag); Mouse DR6/TNFRSF21 Protein (His Tag); AA959878 Protein; R74815 Protein; TR7 Protein; tumor necrosis factor receptor superfamily; member 21; TNFRSF21 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His349

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 42
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse TNFRSF21 (NP_848704.1) extracellular domain (Met 1-His 349) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized mouse TNFRSF21-His at 10ug/ml (100uL/well) can bind biotinylated human APP-Fc with a linear range of 0.31-5ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFRSF21 active protein

Background: TNFRSF21 (death receptor-6, DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain, and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production, suggested that DR6 serves as an important regulatory molecule in T-helper cell activation, and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs, heart, brain and pancreas. Some tumor cells overexpress DR6, typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion, which is subject to future investigations.

Product Categories/Family for TNFRSF21 active protein

Death Receptor Signaling

References

Pan G, et al. (1998) Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 431(3): 351-6.Benschop R, et al. (2009) Tumor necrosis factor receptor superfamily member 21: TNFR-related death receptor-6, DR6. Adv Exp Med Biol. 647: 186-94.Klma M, et al. (2009) Functional analysis of the posttranslational modifications of the death receptor 6. Biochim Biophys Acta. 1793(10): 1579-87.

NCBI and Uniprot Product Information

• NCBI GI #: 30519885
• NCBI GeneID: 94185
• NCBI Accession #: NP_848704.1
• NCBI GenBank Nucleotide #: NM_178589.3
• UniProt Accession #: Q9EPU5; Q3UYG3; Q543Y9; Q91W77; Q91XH9
• Molecular Weight: 71,983 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 21
• NCBI Official Symbol: Tnfrsf21
• NCBI Official Synonym Symbols: DR6; TR7; R74815; AA959878
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21; Death receptor 6; TNFR-related death receptor 6; TNFR-related death receptor-6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf21
• UniProt Synonym Gene Names: Dr6
• UniProt Entry Name: TNR21_MOUSE

Uniprot Description

DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.

Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis

Cellular Component: membrane; axon; integral to plasma membrane; integral to membrane; plasma membrane; intrinsic to plasma membrane

Biological Process: myelination; neuron apoptosis; adaptive immune response; negative regulation of T cell proliferation; negative regulation of myelination; apoptosis; immune system process; regulation of oligodendrocyte differentiation; B cell apoptosis; negative regulation of B cell proliferation; signal transduction; T cell receptor signaling pathway; humoral immune response