SDS-Page
TIE2 Active Protein | TEK active protein
TIE2 Protein, Mouse, Recombinant (ECD, hFc Tag)
Purity
>95% as determined by SDS-PAGE
Synonyms
TIE2; TIE2 Protein; Mouse; Recombinant (ECD; hFc Tag); Mouse Tie2/CD202b/TEK Protein (ECD; Fc Tag); AA517024 Protein; Cd202b Protein; Hyk Protein; STK1 Protein; Tie-2 Protein; Tie2 Protein; TEK tyrosine kinase; endothelial; TEK active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Lys744
Species
Mouse
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Val 19
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the mouse Tek (NP_038718.2)(Met1-Lys744) was expressed with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse ANGPT2 at 10ug/mL (100uL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34ug/mL.
2. Immobilized mouse ANGPT2 at 10ug/mL (100uL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34ug/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Testing Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse ANGPT2 at 10 ug/mL (100 uL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34 ug/mL.)
Testing Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse ANGPT2 at 10 ug/mL (100 uL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34 ug/mL.)
Related Product Information for TEK active protein
Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Product Categories/Family for TEK active protein
References
Jones N, et al. (1998) The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK/TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.
NCBI and Uniprot Product Information
Molecular Weight
The recombinant mouse Tek consists 964 amino acids and predicts a molecular mass of 107.7kDa.
Protein Family
Similar Products
Product Notes
The TEK (Catalog #AAA8123111) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Lys74 4. It is sometimes possible for the material contained within the vial of "TIE2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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