Periostin Active Protein | POSTN active protein

Periostin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122748

• Gene Names: Postn; PN; PLF; Osf2; OSF-2; AI747096; A630052E07Rik
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Periostin; Periostin Protein; Mouse; Recombinant (His Tag); Mouse Periostin/POSTN Protein (His Tag); A630052E07Rik Protein; AI747096 Protein; OSF-2 Protein; Osf2 Protein; peri Protein; PLF Protein; PN Protein; periostin; osteoblast specific factor; POSTN active protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln811

• Species: Mouse
• Activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10ug/mL, 100uL/well), approximately >40% will adhere specifically after 30 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of mouse POSTN (NP_056599.1)(Met 1-Gln 811) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for POSTN active protein

Background: Periostin (POSTN), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.

References

Kudo,Y. et al., 2007, Histol Histopathol. 22 (10):1167-1174. Li, J.S. et al., 2007, World J Gastroenterol. 13 (39): 5261-5266. Oku, E. et al., 2008, Int J Hematol. 88 (1): 57-63. Hamilton, D.W. et al., 2008, J Cell Commun Signal. 2(1-2):9-17. Puglisi, F.J et al., 2008, Clin Pathol. 61 (4): 494-498. Conway, S. J. et al., 2008, Curr Genomics. 9 (8): 548-555.

NCBI and Uniprot Product Information

• NCBI GI #: 7657429
• NCBI GeneID: 50706
• NCBI Accession #: NP_056599.1
• NCBI GenBank Nucleotide #: NM_015784.3
• UniProt Accession #: Q62009; Q8BMJ6; Q8K1K0
• Molecular Weight: 87,099 Da
• NCBI Official Full Name: periostin isoform 1
• NCBI Official Synonym Full Names: periostin, osteoblast specific factor
• NCBI Official Symbol: Postn
• NCBI Official Synonym Symbols: PN; PLF; Osf2; OSF-2; AI747096; A630052E07Rik
• NCBI Protein Information: periostin
• UniProt Protein Name: Periostin
• Protein Family: Periostin
• UniProt Gene Name: Postn
• UniProt Synonym Gene Names: Osf2; PN; OSF-2
• UniProt Entry Name: POSTN_MOUSE

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region; trans-Golgi network

Molecular Function: heparin binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion