Allergin 1 Cell Lysate | MILR1 cell lysate
Human Allergin 1 HEK293 Overexpression Lysate
Gene Names
MILR1; MCA32; MCA-32; C17orf60; Allergin-1
Applications
Western Blot
Synonyms
Allergin 1; Human Allergin 1 HEK293 Overexpression Lysate; Human MILR1/Allergin 1 HEK293 Cell Lysate (WB positive control); mast cell immunoglobulin-like receptor 1; Human Allergin-1 Overexpression Lysate; Human C17orf60 Overexpression Lysate; Human MCA-32 Overexpression Lysate; Human MCA32 Overexpression Lysate; MILR1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
219aa
Sequence
Met1-Lys227
Applicable Applications for MILR1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human MILR1 (NP_001078892.1) (Met1-Lys227) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MILR1 cell lysate
MILR1 (Mast Cell Immunoglobulin Like Receptor 1, also known as MCA32) is a Protein Coding gene. It is broadly expressed in the appendix, lymph node, and other tissues. Cell surface immunoreceptor MILR1 has been shown to suppress immunoglobulin E (IgE)-mediated, mast cell-dependent responses in both mice and humans. The C allele of rs6504230 was associated with increased expression of MILR1, which was following the results of expression quantitative trait loci analysis using human leukocytes. The rs6504230 polymorphism affects MILR1 expression levels in humans, leading to a susceptibility to producing specific IgE antibodies against common allergens. Diseases associated with MILR1 include Mitochondrial Dna Depletion Syndrome 16 and Autosomal Dominant Progressive External Ophthalmoplegia.
This Human Allergin 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Allergin 1 protein from the overexpression lysate was verified.
This Human Allergin 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Allergin 1 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,735 Da
NCBI Official Full Name
allergin-1 isoform Allergin-1L
NCBI Official Synonym Full Names
mast cell immunoglobulin-like receptor 1
NCBI Official Symbol
MILR1
NCBI Official Synonym Symbols
MCA32; MCA-32; C17orf60; Allergin-1
NCBI Protein Information
allergin-1; mast cell antigen 32; allergy inhibitory receptor 1; probable mast cell antigen 32 homolog
UniProt Protein Name
Allergin-1
Protein Family
UniProt Gene Name
MILR1
UniProt Synonym Gene Names
C17orf60; MCA32; MCA-32
UniProt Entry Name
MILR1_HUMAN
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Product Notes
The MILR1 milr1 (Catalog #AAA8115649) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 219aa. AAA Biotech's Allergin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MILR1 milr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys22 7. It is sometimes possible for the material contained within the vial of "Allergin 1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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