Interleukin-12 Active Protein | mIL 12 active protein

Recombinant Mouse Interleukin-12

Cat. Num. AAA145318

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Interleukin-12; Recombinant Mouse Interleukin-12; IL 12 Mouse; Interleukin-12 Mouse Recombinant; NKSF; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor (CLMF); TSF; Edodekin-alpha; IL-12; mIL 12 active protein

• Host: HEK293 Cells
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: The protein was lyophilized from 0.5xPBS, pH 7.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSAp40 Subunit:MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
• Sequence Length: 253

• Solubility: It is recommended to reconstitute the lyophilized murine IL12 in sterile distilled pyrogen free water at 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.
• Preparation and Storage: Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18 degree C. Upon reconstitution IL12 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mIL 12 active protein

Description: Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.

Introduction: IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.

Product Categories/Family for mIL 12 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 4323579
• NCBI GeneID: 3592
• Molecular Weight: 28,293 Da
• NCBI Official Full Name: interleukin 12
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• UniProt Protein Name: Interleukin 12
• Protein Family: Interleukin
• UniProt Gene Name: IL12A
• UniProt Entry Name: O60595_HUMAN

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]