CCK4/PTK7 Lysate | PTK7 lysate

Cynomolgus, Rhesus CCK4/PTK7 HEK293 Overexpression Lysate

• Applications: Western Blot
• Synonyms: CCK4/PTK7; Cynomolgus; Rhesus CCK4/PTK7 HEK293 Overexpression Lysate; Cynomolgus/Rhesus CCK4/PTK7 HEK293 Cell Lysate (WB positive control); PTK7 lysate

• Host: HEK293 Cells
• Form/Format: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.; 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

• Applicable Applications for PTK7 lysate: Western Blot (WB)
• Species: Cynomolgus, Rhesus
• Sequence Information: A DNA sequence encoding the cynomolgus/rhesus PTK7 (XP_005552998.1/NP_001248508.1)(Met1-Thr704) was expressed with a polyhistidine tag at the C-terminus.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.; 2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.; 3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
• Preparation and Storage: Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Related Product Information for PTK7 lysate

Description: This Cynomolgus, Rhesus CCK4/PTK7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CCK4/PTK7 protein from the overexpression lysate was verified.

Background: Protein tyrosine kinase 7 (PTK7) is a highly conserved but catalytically inactive receptor tyrosine kinase in the Wnt signaling pathway. PTK7 regulates various processes in embryonic development and tissue homeostasis. On a cellular level PTK7 affects the establishment of cell polarity, the regulation of cell movement and migration as well as cell invasion. The PTK7 receptor interacts with ligands, co-receptors, and intracellular transducers of Wnt signaling pathways, pointing to a function in the fine-tuning of the Wnt signaling network. PTK7 is a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. PTK7 plays an important role in human cancers, including triple-negative breast cancer (TNBC), ovarian cancer, and non-small cell lung cancer (NSCLC).

References

Lichtig H, et al. (2019) Ptk7 proteolytic fragment proteins function during early xenopus development. Dev Biol 453 (1): 48-55.Damelin M, et al. (2017) A ptk7-targeted antibody-drug conjugate reduces tumor-initiating cells and induces sustained tumor regressions. Sci Transl Med 9 (372): eaag2611.Berger H, et al. (2017) Ptk7 faces the wnt in development and disease. Front Cell Dev Biol 5 31.