IL-12 Cell Lysate | IL12A cell lysate

Human IL-12 HEK293 Overexpression Lysate

Cat. Num. AAA8118128

• Gene Names: IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
• Applications: Western Blot
• Synonyms: IL-12; Human IL-12 HEK293 Overexpression Lysate; Human IL-12 (IL12A & IL12B Heterodimer) HEK293 Cell Lysate (WB positive control); interleukin 12A; Human CLMF Overexpression Lysate; Human CLMF2 Overexpression Lysate; Human IL-12A Overexpression Lysate; Human IL-12B Overexpression Lysate; Human IL12 Overexpression Lysate; Human IL12 p70 Overexpression Lysate; Human IMD28 Overexpression Lysate; Human IMD29 Overexpression Lysate; Human NFSK Overexpression Lysate; Human NKSF Overexpression Lysate; Human NKSF1 Overexpression Lysate; Human NKSF2 Overexpression Lysate; Human P35 Overexpression Lysate; IL12A cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 525 (208 + 317)aa
• Sequence: Met1-Ser219, Met1-Ser328

Applicable Applications for IL12A cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.