Insulin Like Growth Factor Binding Protein-4 Active Protein | IGFBP4 active protein

Recombinant Human Insulin Like Growth Factor Binding Protein-4

Cat. Num. AAA144993

• Gene Names: IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor Binding Protein-4; Recombinant Human Insulin Like Growth Factor Binding Protein-4; IGFBP 4 Human; Insulin-Like Growth Factor Binding Protein-4 Human; Insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4; IGFBP-4; IGFBP4; IBP4; BP-4; HT29-IGFBP; IGFBP4 active protein

• Host: HEK293 Cells
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: IGFBP 4 was lyophilized after extensive dialysis against PBS.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag
• Sequence Length: 258

• Solubility: It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 range is 0.01-0.09 ug/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.
• Preparation and Storage: Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGFBP-4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IGFBP4 active protein

Description: IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions.IGFBP4 is purified by proprietary chromatographic techniques.

Introduction: Insulin-like growth factor-binding protein 4 (IGFBP-4) belongs to the insulin-like growth factor binding protein (IGFBP) family. IGFBP4 includes an IGFBP domain and a thyroglobulin type-I domain. IGFBP4 binds both insulin-like growth factors (IGFs) I and II. IGFBP-4 circulates in the plasma in both glycosylated and non-glycosylated forms. IGFBPs can either inhibit or enhance the biological activities of IGF, or act in an IGF independent manner. IGFBP-4 is exceptional since it consistently inhibits several cancer cells in vivo and in vitro, suggesting that it may function as an apoptotic factor. IGFBP4 is produced by all colon cancer cells. Binding of IGFBP-4 prolongs the half-life of the IGFs and changes their interaction with cell surface receptors.

Product Categories/Family for IGFBP4 active protein

Cytokines and Growth Factors; Growth Factors; IGFBP

NCBI and Uniprot Product Information

• NCBI GI #: 62243290
• NCBI GeneID: 3487
• NCBI Accession #: NP_001543.2
• NCBI GenBank Nucleotide #: NM_001552.2
• UniProt Accession #: P22692; Q5U012; Q9UCL6; A0N9W2; B4E351
• Molecular Weight: 17,635 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 4
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 4
• NCBI Official Symbol: IGFBP4
• NCBI Official Synonym Symbols: BP-4; IBP4; IGFBP-4; HT29-IGFBP
• NCBI Protein Information: insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein 4
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP4
• UniProt Synonym Gene Names: IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
• UniProt Entry Name: IBP4_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; cellular protein metabolic process; positive regulation of MAPKKK cascade; regulation of cell growth; signal transduction; inflammatory response; DNA metabolic process; skeletal development