CD23 Active Protein | FCER2 active protein

Human CellExp CD23, human recombinant

Cat. Num. AAA844916

• Gene Names: FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: CD23; Human CellExp CD23; human recombinant; FCER2; FCER-2; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcepsilonRII; Fc epsilon RII; FCER2 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 320

• Applicable Applications for FCER2 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 mug/ml (100 mul/well) can bind human IgE with a linear range of 0.01-1.2 mug/ml.
• Results: Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 mug/ml (100 mul/well) can bind human IgE with a linear range of 0.01-1.2 mug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for FCER2 active protein

Background: Cluster of differentiation 23 (CD23), also known as Low affinity immunoglobulin epsilon Fc receptor (FCER2), C-type lectin domain family 4 member J (CLEC4J), Fc-epsilon-RII (FcepsilonRII), Immunoglobulin E-binding factor (IGEBF), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have role of transportation in antibody feedback regulation. Antigen that enters the blood stream is captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative).

Product Categories/Family for FCER2 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 334085205
• NCBI GeneID: 2208
• NCBI Accession #: NP_001193948.2
• NCBI GenBank Nucleotide #: NM_001207019.2
• UniProt Accession #: P06734
• Molecular Weight: This protein is fused with a fused with 6×his tag at the N-terminus and has a calculated MW of 33.2 kDa expressed. The predicted N-terminus is Asp48. Protein migrates as 40-44 kDa in reduced SDS-PAGE resulting from glycosylation.
• NCBI Official Full Name: low affinity immunoglobulin epsilon Fc receptor isoform b
• NCBI Official Synonym Full Names: Fc fragment of IgE, low affinity II, receptor for (CD23)
• NCBI Official Symbol: FCER2
• NCBI Official Synonym Symbols: CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• Protein Family: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: FCER2
• UniProt Synonym Gene Names: CD23A; CLEC4J; FCE2; IGEBF
• UniProt Entry Name: FCER2_HUMAN

NCBI Description

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).