ENPP-5 Cell Lysate | ENPP5 cell lysate
Human ENPP-5 HEK293 Overexpression Lysate
Gene Names
ENPP5; KIAA0879
Applications
Western Blot
Synonyms
ENPP-5; Human ENPP-5 HEK293 Overexpression Lysate; Human ENPP5 HEK293 Cell Lysate (WB positive control); ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative); Human NPP-5 Overexpression Lysate; ENPP5 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
416aa
Sequence
Met1-Gly429
Applicable Applications for ENPP5 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ENPP5 (Q9UJA9) (Met1-Gly429) with a C-terminal polyhistidine tag was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ENPP5 cell lysate
ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family (NPP). It is a family comprised of dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. However, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.
This Human ENPP-5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ENPP-5 protein from the overexpression lysate was verified.
This Human ENPP-5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ENPP-5 protein from the overexpression lysate was verified.
Product Categories/Family for ENPP5 cell lysate
References
Strausberg RL, et al. (2002) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10, 000 human genes. Genomics. 89(3):307-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,666 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 5
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative)
NCBI Official Symbol
ENPP5
NCBI Official Synonym Symbols
KIAA0879
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 5; NPP-5; E-NPP 5; OTTHUMP00000016542; OTTHUMP00000016543; ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function)
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5
Protein Family
UniProt Gene Name
ENPP5
UniProt Entry Name
ENPP5_HUMAN
NCBI Description
This gene encodes a type-I transmembrane glycoprotein. Studies in rat suggest the encoded protein may play a role in neuronal cell communications. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2014]
Uniprot Description
ENPP5: May play a role in neuronal cell communication. Lacks nucleotide pyrophosphatase and lysopholipase D activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Membrane protein, integral; EC 3.1.-.-; Motility/polarity/chemotaxis; Hydrolase
Chromosomal Location of Human Ortholog: 6p21.1-p11.2
Cellular Component: plasma membrane; extracellular region; integral to membrane
Molecular Function: hydrolase activity; metal ion binding
Biological Process: metabolic process; cell communication
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Product Notes
The ENPP5 enpp5 (Catalog #AAA8114560) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 416aa. AAA Biotech's ENPP-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ENPP5 enpp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly42 9. It is sometimes possible for the material contained within the vial of "ENPP-5, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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