Cathepsin D Active Protein | CTSD active protein

Human CellExp Cathepsin D, human recombinant

Cat. Num. AAA844250

• Gene Names: CTSD; CPSD; CLN10; HEL-S-130P
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Cathepsin D; Human CellExp Cathepsin D; human recombinant; CTSD; CPSD; CLN10; CTSD active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 412

• Applicable Applications for CTSD active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >450 pmol/min/ug.
• Results: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >450 pmol/min/ug.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CTSD active protein

Background: Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Product Categories/Family for CTSD active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Proteases; CathepsinsApoptosis & Related Products; Non-Caspase Proteases; Cathepsin; Cathepsin Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4503143
• NCBI GeneID: 1509
• NCBI Accession #: NP_001900.1
• NCBI GenBank Nucleotide #: NM_001909.4
• UniProt Accession #: P07339; Q6IB57
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 43.6 kDa. The predicted N-terminus is Ser 19. DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.
• NCBI Official Full Name: cathepsin D preproprotein
• NCBI Official Synonym Full Names: cathepsin D
• NCBI Official Symbol: CTSD
• NCBI Official Synonym Symbols: CPSD; CLN10; HEL-S-130P
• NCBI Protein Information: cathepsin D
• UniProt Protein Name: Cathepsin D
• Protein Family: Cathepsin
• UniProt Gene Name: CTSD
• UniProt Synonym Gene Names: CPSD
• UniProt Entry Name: CATD_HUMAN

NCBI Description

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.

Protein type: Protease; Autophagy; Motility/polarity/chemotaxis; EC 3.4.23.5

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; lysosome; melanosome; extracellular region

Molecular Function: protein binding; aspartic-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10