Carboxypeptidase B2 Cell Lysate | CPB2 cell lysate
Mouse Carboxypeptidase B2 HEK293 Overexpression Lysate
Gene Names
Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
Applications
Western Blot
Synonyms
Carboxypeptidase B2; Mouse Carboxypeptidase B2 HEK293 Overexpression Lysate; Mouse Carboxypeptidase B2/CPB2 HEK293 Cell Lysate (WB positive control); carboxypeptidase B2 (plasma); Mouse 1110032P04Rik Overexpression Lysate; Mouse 4930405E17Rik Overexpression Lysate; Mouse AI255929 Overexpression Lysate; Mouse CPR Overexpression Lysate; Mouse Cpu Overexpression Lysate; Mouse TAFI Overexpression Lysate; CPB2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
412aa
Sequence
Met1-Thr422
Applicable Applications for CPB2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of mouse CPB2 (Q9JHH6) (Met 1-Thr 422) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CPB2 cell lysate
Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
This Mouse Carboxypeptidase B2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carboxypeptidase B2 protein from the overexpression lysate was verified.
This Mouse Carboxypeptidase B2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Carboxypeptidase B2 protein from the overexpression lysate was verified.
References
Eaton DL. et al., 1991, J Biol Chem. 266 (32): 21833-8. Boffa MB. et al., 1999, Biochemistry. 38 (20): 6547-58. Liu T. et al., 2005, J. Proteome Res. 4: 2070-80. Valnickova Z. et al., 2006, Biochemistry. 45: 1525-35. Chen R. et al., 2009, J. Proteome Res. 8: 651-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,871 Da
NCBI Official Full Name
thrombin-activatable fibrinolysis inhibitor
NCBI Official Synonym Full Names
carboxypeptidase B2 (plasma)
NCBI Official Symbol
Cpb2
NCBI Official Synonym Symbols
CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
NCBI Protein Information
carboxypeptidase B2; carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
UniProt Protein Name
Carboxypeptidase B2
Protein Family
UniProt Gene Name
Cpb2
UniProt Synonym Gene Names
Tafi; CPR; CPU; TAFI
UniProt Entry Name
CBPB2_MOUSE
Uniprot Description
CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.20; Secreted; Protease; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; hydrolase activity; carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity
Biological Process: fibrinolysis; negative regulation of fibrinolysis; hemostasis; response to heat; proteolysis; blood coagulation
Research Articles on CPB2
Similar Products
Product Notes
The CPB2 cpb2 (Catalog #AAA8116959) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 412aa. AAA Biotech's Carboxypeptidase B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CPB2 cpb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr42 2. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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