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COVID 19 Spike S1 Coronavirus Active Protein | COVID-19 active protein
Recombinant 2019-nCoV Spike S1 Protein with His tag
Purity
>90% by SDS-PAGE; > 95% by HPLC
Synonyms
COVID 19 Spike S1 Coronavirus; Recombinant 2019-nCoV Spike S1 Protein with His tag; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1 Protein; COVID-19 active protein
Host
HEK293 Cells
Purity/Purification
>90% by SDS-PAGE; > 95% by HPLC
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
2019-nCoV
Endotoxin
< 1.0 EU/ug of the protein by LAL method.
Tag
6xHis tag at the C-terminus
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.|After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
SDS-PAGE (SDS)
(Recombinant 2019-nCoV Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.)
Testing Data
(Immobilized Recombinant 2019-nCoV Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.)
Testing Data
(The purity of 2019-nCoV Spike S1 Protein with His tag (MBS9141943) was greater than 95% as determined by SEC-HPLC.)
Testing Data
(Immobilized Human ACE2 on COOH Chip, can bind 2019-nCoV Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay (Nicoya OpenSPR).)
Related Product Information for COVID-19 active protein
Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.
Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing antibody and T-cell responses, as well as protective immunity.
Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing antibody and T-cell responses, as well as protective immunity.
