Zinc Alpha 2 Glycoprotein/AZGP1 Cell Lysate | AZGP1 cell lysate
Human Zinc Alpha 2 Glycoprotein/AZGP1 HEK293 Overexpression Lysate
Gene Names
AZGP1; ZAG; ZA2G
Applications
Western Blot
Synonyms
Zinc Alpha 2 Glycoprotein/AZGP1; Human Zinc Alpha 2 Glycoprotein/AZGP1 HEK293 Overexpression Lysate; Human Alpha-2-glycoprotein/AZGP1 HEK293 Cell Lysate (WB positive control); alpha-2-glycoprotein 1; zinc-binding; Human ZA2G Overexpression Lysate; Human ZAG Overexpression Lysate; AZGP1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
289aa
Sequence
Met1-Ser298
Applicable Applications for AZGP1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human AZGP1 (P25311) (Met1-Ser298) with a C-terminal polyhistidine tag was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for AZGP1 cell lysate
Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.
This Human Zinc Alpha 2 Glycoprotein/AZGP1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Zinc Alpha 2 Glycoprotein/AZGP1 protein from the overexpression lysate was verified.
This Human Zinc Alpha 2 Glycoprotein/AZGP1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Zinc Alpha 2 Glycoprotein/AZGP1 protein from the overexpression lysate was verified.
References
Tada T, et al. (1991) Immunohistochemical localization of Zn-alpha 2-glycoprotein in normal human tissues. J Histochem Cytochem. 39(9):1221-6.Vanni H, et al. (2009) Cigarette Smoking Induces Overexpression of a Fat-Depleting Gene AZGP1 in the Human. Chest. 135(5):1197-208.Ueyama H, et al. (1991) Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene. Biochem Biophys Res Commun. 177(2):696-703.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,259 Da
NCBI Official Full Name
zinc-alpha-2-glycoprotein
NCBI Official Synonym Full Names
alpha-2-glycoprotein 1, zinc-binding
NCBI Official Symbol
AZGP1
NCBI Official Synonym Symbols
ZAG; ZA2G
NCBI Protein Information
zinc-alpha-2-glycoprotein; zn-alpha-2-GP; Zn-alpha2-glycoprotein; zn-alpha-2-glycoprotein; Alpha-2-glycoprotein, zinc
UniProt Protein Name
Zinc-alpha-2-glycoprotein
Protein Family
UniProt Gene Name
AZGP1
UniProt Synonym Gene Names
ZAG; ZNGP1; Zn-alpha-2-GP
UniProt Entry Name
ZA2G_HUMAN
Uniprot Description
AZGP1: Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. Belongs to the MHC class I family.
Protein type: Cell adhesion; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: extracellular space; plasma membrane; extracellular region; nucleus; MHC class I protein complex
Molecular Function: peptide antigen binding; protein transmembrane transporter activity; glycoprotein binding; antigen binding; ribonuclease activity
Biological Process: negative regulation of cell proliferation; antigen processing and presentation; retinal homeostasis; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; immune response; detection of chemical stimulus involved in sensory perception of bitter taste; cell adhesion
Research Articles on AZGP1
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Product Notes
The AZGP1 azgp1 (Catalog #AAA8115098) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 289aa. AAA Biotech's Zinc Alpha 2 Glycoprotein/AZGP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the AZGP1 azgp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser29 8. It is sometimes possible for the material contained within the vial of "Zinc Alpha 2 Glycoprotein/AZGP1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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