TM4SF2/TSPAN7 Cell Lysate | TSPAN7 cell lysate
Mouse TM4SF2/TSPAN7 HEK293 Overexpression Lysate
Gene Names
Tspan7; A15; Mxs1; PE31; Cd231; TALLA; R74651; Tm4sf2; AI323365; 1200014P11Rik
Applications
Western Blot
Synonyms
TM4SF2/TSPAN7; Mouse TM4SF2/TSPAN7 HEK293 Overexpression Lysate; Mouse TALLA-1/TSPAN7 HEK293 Cell Lysate (WB positive control); tetraspanin 7; Mouse 1200014P11Rik Overexpression Lysate; Mouse A15 Overexpression Lysate; Mouse AI323365 Overexpression Lysate; Mouse Cd231 Overexpression Lysate; Mouse Mxs1 Overexpression Lysate; Mouse PE31 Overexpression Lysate; Mouse R74651 Overexpression Lysate; Mouse TALLA Overexpression Lysate; Mouse Tm4sf2 Overexpression Lysate; TSPAN7 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
112aa
Sequence
Arg113-Met213
Applicable Applications for TSPAN7 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse TSPAN7 (Q62283) (Arg113-Met213) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TSPAN7 cell lysate
TALLA-1, also known as TSPAN7, is a member of the transmembrane 4 superfamily Most members of this family are cell-surface proteins that are characterized by the presence of four hydrophobic domains. TALLA-1 gene is associated with X-linked mental retardation and neuropsychiatric diseases such as Huntington's chorea, fragile X syndrome and myotonic dystrophy. TALLA-1 is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. It is known to complex with integrins.
This Mouse TM4SF2/TSPAN7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TM4SF2/TSPAN7 protein from the overexpression lysate was verified.
This Mouse TM4SF2/TSPAN7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TM4SF2/TSPAN7 protein from the overexpression lysate was verified.
References
Berditchevski F. 2002, J Cell Sci. 114 (23): 4143-51. Castellvi-Bel S. et al., 2001, Mol Genet Metab. 72 (2): 104-8. Abidi FE. et al., 2002, J Med Genet. 39 (6): 430-3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,544 Da
NCBI Official Full Name
tetraspanin-7
NCBI Official Synonym Full Names
tetraspanin 7
NCBI Official Symbol
Tspan7
NCBI Official Synonym Symbols
A15; Mxs1; PE31; Cd231; TALLA; R74651; Tm4sf2; AI323365; 1200014P11Rik
NCBI Protein Information
tetraspanin-7; tspan-7; TALLA homolog; cell surface glycoprotein A15; transmembrane 4 superfamily member 2
UniProt Protein Name
Tetraspanin-7
UniProt Gene Name
Tspan7
UniProt Synonym Gene Names
Mxs1; Tm4sf2; Tspan-7
UniProt Entry Name
TSN7_MOUSE
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Product Notes
The TSPAN7 tspan7 (Catalog #AAA8116912) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 112aa. AAA Biotech's TM4SF2/TSPAN7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TSPAN7 tspan7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg113-Met 213. It is sometimes possible for the material contained within the vial of "TM4SF2/TSPAN7, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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