REG2 cell lysate
Mouse REG2 HEK293 Overexpression Lysate
Gene Names
Reg2; RegII
Applications
Western Blot
Synonyms
REG2; Mouse REG2 HEK293 Overexpression Lysate; Mouse REG2/REG-2 HEK293 Cell Lysate (WB positive control); regenerating islet-derived 2; Mouse REG2 Overexpression Lysate; Mouse RegII Overexpression Lysate; REG2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
162aa
Sequence
Met1-Ala173
Applicable Applications for REG2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse REG2 (NP_033069.1) (Met1-Ala173) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for REG2 cell lysate
Regenerating islet-derived proteins (Regs) comprise the superfamily of C-type lectin proteins encoded by Reg1, Reg2, Reg3a, Reg3b, Reg3g, Reg3d, and Reg4. The Reg family proteins are expressed in various tissues and have pleiotropic functions. Reg3b and Reg3g encode murine Reg3beta and Reg3gamma, respectively, and are murine homolog of human REG3A. Both proteins are highly expressed in the small intestine of adult mice at both mRNA and protein levels, but their expression is very low in the colon. Intriguingly, expression of Reg3beta and Reg3gamma is not detectable in the embryonic mouse intestine but gradually increases along with colonization of the commensal bacteria after birth.
This Mouse REG2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of REG2 protein from the overexpression lysate was verified.
This Mouse REG2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of REG2 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,407 Da
NCBI Official Full Name
lithostathine-2
NCBI Official Synonym Full Names
regenerating islet-derived 2
NCBI Official Symbol
Reg2
NCBI Official Synonym Symbols
RegII
NCBI Protein Information
lithostathine-2; PSP; PTP; REG-2; pancreatic stone protein 2; pancreatic thread protein 2; islet of Langerhans regenerating protein 2; regenerating islet-derived, mouse homolog 2
UniProt Protein Name
Lithostathine-2
Protein Family
UniProt Gene Name
Reg2
UniProt Synonym Gene Names
REG-2; PSP; PTP
UniProt Entry Name
LIT2_MOUSE
Uniprot Description
Function: Might act as an inhibitor of spontaneous calcium carbonate precipitation.
Subcellular location: Secreted
By similarity.
Tissue specificity: Expressed only in regenerating islets and normal exocrine pancreas, but not in normal pancreatic islets. Expressed strongly in pancreas, weakly in liver, but not at all in gall bladder.
Sequence similarities: Contains 1 C-type lectin domain.
Research Articles on REG2
Similar Products
Product Notes
The REG2 reg2 (Catalog #AAA8117096) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 162aa. AAA Biotech's REG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the REG2 reg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala17 3. It is sometimes possible for the material contained within the vial of "REG2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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