PSG1 cell lysate
Human PSG1 HEK293 Overexpression Lysate
Gene Names
PSG1; SP1; B1G1; PBG1; CD66f; PSBG1; PSG95; PSGGA; DHFRP2; PSBG-1; PSGIIA; FL-NCA-1/2; PS-beta-C/D; PS-beta-G-1
Applications
Western Blot
Synonyms
PSG1; Human PSG1 HEK293 Overexpression Lysate; Human Pregnancy Specific Glycoprotein 1 HEK293 Cell Lysate (WB positive control); pregnancy specific beta-1-glycoprotein 1; Human B1G1 Overexpression Lysate; Human CD66f Overexpression Lysate; Human DHFRP2 Overexpression Lysate; Human FL-NCA-1/2 Overexpression Lysate; Human PBG1 Overexpression Lysate; Human PS-beta-C/D Overexpression Lysate; Human PS-beta-G-1 Overexpression Lysate; Human PSBG-1 Overexpression Lysate; Human PSBG1 Overexpression Lysate; Human PSG95 Overexpression Lysate; Human PSGGA Overexpression Lysate; Human PSGIIA Overexpression Lysate; Human SP1 Overexpression Lysate; PSG1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
623aa
Sequence
Met1-Pro419
Applicable Applications for PSG1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human PSG1 (NP_001171754.1) (Met1-Pro419) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PSG1 cell lysate
Pregnancy-specific glycoproteins (PSGs) are a family of Ig-like proteins secreted by specialized placental cells. The PSG1 structure is composed of a single Ig variable region-like N-terminal domain and three Ig constant region-like domains termed A1, A2, and B2. Members of the human and murine PSG family have been shown to induce anti-inflammatory cytokines from monocytes and macrophages and to stimulate angiogenesis. Two distinct domains of PSG1 are involved in the regulation of TGF-beta1 and provide a mechanistic framework for how PSGs modulate the immunoregulatory environment at the maternal-fetal interface for successful pregnancy outcome.
This Human PSG1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PSG1 protein from the overexpression lysate was verified.
This Human PSG1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PSG1 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,866 Da
NCBI Official Full Name
pregnancy-specific beta-1-glycoprotein 1 isoform 2
NCBI Official Synonym Full Names
pregnancy specific beta-1-glycoprotein 1
NCBI Official Symbol
PSG1
NCBI Official Synonym Symbols
SP1; B1G1; PBG1; CD66f; PSBG1; PSG95; PSGGA; DHFRP2; PSBG-1; PSGIIA; FL-NCA-1/2; PS-beta-C/D; PS-beta-G-1
NCBI Protein Information
pregnancy-specific beta-1-glycoprotein 1
UniProt Protein Name
Pregnancy-specific beta-1-glycoprotein 1
Protein Family
UniProt Gene Name
PSG1
UniProt Synonym Gene Names
B1G1; PSBG1; PSGGA; PS-beta-G-1; PSBG-1; Pregnancy-specific glycoprotein 1; FL-NCA-1/2; PS-beta-C/D
UniProt Entry Name
PSG1_HUMAN
NCBI Description
The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women (Horne et al., 1976 [PubMed 971765]). PSG is a member of the immunoglobulin (Ig) superfamily (Watanabe and Chou, 1988 [PubMed 3257488]; Streydio et al., 1988 [PubMed 3260773]).[supplied by OMIM, Oct 2009]
Uniprot Description
PSG1: Belongs to the immunoglobulin superfamily. CEA family. 4 isoforms of the human protein are produced by alternative splicing
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: extracellular region
Biological Process: female pregnancy
Research Articles on PSG1
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Product Notes
The PSG1 psg1 (Catalog #AAA8115478) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 623aa. AAA Biotech's PSG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PSG1 psg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro41 9. It is sometimes possible for the material contained within the vial of "PSG1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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