IL12RB2 cell lysate
Mouse IL12RB2 HEK293 Overexpression Lysate
Gene Names
Il12rb2; Ifnm; IL-12RB2; A930027I18Rik
Applications
Western Blot
Synonyms
IL12RB2; Mouse IL12RB2 HEK293 Overexpression Lysate; Mouse IL12RB2/IL12R-beta 2 HEK293 Cell Lysate (WB positive control); interleukin 12 receptor; beta 2; Mouse A930027I18Rik Overexpression Lysate; Mouse Ifnm Overexpression Lysate; Mouse IL-12RB2 Overexpression Lysate; IL12RB2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
625aa
Sequence
Met1-Asn637
Applicable Applications for IL12RB2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse IL12RB2 (NP_032380.1) extracellular domain (Met 1-Asn 637) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL12RB2 cell lysate
Interleukin-12 receptor subunit beta-2 (IL12RB2), also known as IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, and IL-12RB2, is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. IL12RB2 belongs to the type I cytokine receptor family. The coexpression of IL12RB2 and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of IL12RB2 is up-regulated by IFN gamma in Th1 cells and plays a role in Th1 cell differentiation. The up-regulation of IL12RB2 is found to be associated with some infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. IL12RB2 promotes the proliferation of T-cells as well as NK cells. IL12RB2 induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
This Mouse IL12RB2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL12RB2 protein from the overexpression lysate was verified.
This Mouse IL12RB2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL12RB2 protein from the overexpression lysate was verified.
Product Categories/Family for IL12RB2 cell lysate
References
Yamamoto K, et al. (1997) Assignment of IL12RB1 and IL12RB2, interleukin-12 receptor beta 1 and beta 2 chains, to human chromosome 19 band p13.1 and chromosome 1 band p31.2, respectively, by in situ hybridization. Cytogenet. 77 (3-4): 257-8.Morton SM, et al. (1998) Assignment of IL12RB2 to human chromosome 1p31.3-->p31.2 between D1S230 and D1S198. Cytogenet. Cell Genet. 79 (3-4): 282-3.Strausberg RL, et al. (2003) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci USA. 99 (26): 16899-903.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,197 Da
NCBI Official Full Name
interleukin-12 receptor subunit beta-2
NCBI Official Synonym Full Names
interleukin 12 receptor, beta 2
NCBI Official Symbol
Il12rb2
NCBI Official Synonym Symbols
Ifnm; IL-12RB2; A930027I18Rik
NCBI Protein Information
interleukin-12 receptor subunit beta-2; IL-12R-beta-2; IL-12R subunit beta-2; IL-12 receptor subunit beta-2; interferon response to microorganisms
UniProt Protein Name
Interleukin-12 receptor subunit beta-2
Protein Family
UniProt Gene Name
Il12rb2
UniProt Synonym Gene Names
IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
UniProt Entry Name
I12R2_MOUSE
Uniprot Description
IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Receptor, cytokine; Membrane protein, integral
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein kinase binding
Biological Process: positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; response to cytokine stimulus; interferon-gamma production; response to lipopolysaccharide
Research Articles on IL12RB2
Similar Products
Product Notes
The IL12RB2 il12rb2 (Catalog #AAA8116378) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 625aa. AAA Biotech's IL12RB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL12RB2 il12rb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn63 7. It is sometimes possible for the material contained within the vial of "IL12RB2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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