GLIPR1 cell lysate
Mouse GLIPR1 HEK293 Overexpression Lysate
Gene Names
Glipr1; RTVP1; RTVP-1; mRTVP-1; 2410114O14Rik
Applications
Western Blot
Synonyms
GLIPR1; Mouse GLIPR1 HEK293 Overexpression Lysate; Mouse GLIPR1/RTVP1 HEK293 Cell Lysate (WB positive control); GLI pathogenesis-related 1; Mouse 2410114O14Rik Overexpression Lysate; Mouse mRTVP-1 Overexpression Lysate; Mouse RTVP-1 Overexpression Lysate; Mouse RTVP1 Overexpression Lysate; GLIPR1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
217aa
Sequence
Met1-Thr223
Applicable Applications for GLIPR1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse GLIPR1 (NP_082884.1) (Met1-Thr223) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GLIPR1 cell lysate
Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to theCRISP family. GLIPR1/RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1/RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1/RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1/RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1/RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1/RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1/RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1/RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1/RTVP-1 is a potential therapeutic target in gliomas.
This Mouse GLIPR1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GLIPR1 protein from the overexpression lysate was verified.
This Mouse GLIPR1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GLIPR1 protein from the overexpression lysate was verified.
References
Murphy E.V., et al., 1995, Gene. 159:131-5. Rich T., et al., 1996, Gene. 180:125-30. Rosenzweig, T. et al., 2006, Cancer Res. 66 (8):4139-48.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,129 Da
NCBI Official Full Name
glioma pathogenesis-related protein 1
NCBI Official Synonym Full Names
GLI pathogenesis-related 1 (glioma)
NCBI Official Symbol
Glipr1
NCBI Official Synonym Symbols
RTVP1; RTVP-1; mRTVP-1; 2410114O14Rik
NCBI Protein Information
glioma pathogenesis-related protein 1
UniProt Protein Name
Glioma pathogenesis-related protein 1
Protein Family
UniProt Gene Name
Glipr1
UniProt Synonym Gene Names
GliPR 1
Uniprot Description
GLIPR1: a protein with similarity to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family. Increased expression of this gene is associated with myelomocytic differentiation in macrophage and decreased expression of this gene through gene methylation is associated with prostate cancer. The protein has proapoptotic activities in prostate and bladder cancer cells. This gene is a member of a cluster on chromosome 12 containing two other similar genes. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10|10 D2
Cellular Component: extracellular region; integral component of membrane; membrane
Research Articles on GLIPR1
Similar Products
Product Notes
The GLIPR1 glipr1 (Catalog #AAA8116909) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 217aa. AAA Biotech's GLIPR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GLIPR1 glipr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr22 3. It is sometimes possible for the material contained within the vial of "GLIPR1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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