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ERBB4 cell lysate

Human ErbB4 HEK293 Overexpression Lysate

Gene Names
ERBB4; HER4; ALS19; p180erbB4
Applications
Western Blot
Synonyms
ErbB4; Human ErbB4 HEK293 Overexpression Lysate; Human/Rhesus HER4/ErbB4 HEK293 Cell Lysate (WB positive control); erb-b2 receptor tyrosine kinase 4; Human ALS19 Overexpression Lysate; Human HER4 Overexpression Lysate; Human p180erbB4 Overexpression Lysate; ERBB4 cell lysate
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For Research Use Only!
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
865aa
Sequence
Met1-Arg649
Applicable Applications for ERBB4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ERBB4 (NP_005226.1) (Met1-Arg649) was expressed, fused with the Fc region of human IgG1 at the C-terminus.Human and Rhesus ERBB4 sequences are identical.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ERBB4 cell lysate
ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

This Human ErbB4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ErbB4 protein from the overexpression lysate was verified.
Product Categories/Family for ERBB4 cell lysate
References
Huang, Y Z, et al. (2000) Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses. Neuron. 26(2):443-55.
Garcia, R A, et al. (2000) The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses. Proc Natl Acad Sci. 97(7):3596-601.
Silberberg G, et al. (2006) The involvement of ErbB4 with schizophrenia: association and expression studies. Am J Med Genet. 141(B2):142-8.
Sardi SP, et al. (2006) Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell. 127(1):185-97.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
143,968 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 4
NCBI Official Symbol
ERBB4
NCBI Official Synonym Symbols
HER4; ALS19; p180erbB4
NCBI Protein Information
receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; human epidermal growth factor receptor 4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblast
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-4
Protein Family
UniProt Gene Name
ERBB4
UniProt Synonym Gene Names
HER4; 4ICD; E4ICD
UniProt Entry Name
ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: nucleoplasm; mitochondrion; basolateral plasma membrane; mitochondrial matrix; plasma membrane; extracellular region; integral to membrane; cytosol; nucleus; receptor complex

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; cell fate commitment; phosphoinositide-mediated signaling; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of protein amino acid phosphorylation; mitochondrial fragmentation during apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Amyotrophic Lateral Sclerosis 19

Research Articles on ERBB4

1. This study provide evidence from this independent Australian postmortem cohort that ErbB4-JMa expression is elevated in schizophrenia.

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Product Notes

The ERBB4 erbb4 (Catalog #AAA8113770) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 865aa. AAA Biotech's ErbB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ERBB4 erbb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg64 9. It is sometimes possible for the material contained within the vial of "ErbB4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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