CEACAM1 cell lysate
Mouse CEACAM1 HEK293 Overexpression Lysate
Gene Names
Ceacam1; Bgp; Cc1; Hv2; Bgp1; Cea1; Cea7; Hv-2; MHVR; bb-1; C-CAM; CD66a; Cea-1; Cea-7; MHVR1; Mhv-1; mCEA1; mmCGM1; mmCGM2; mmCGM1a
Applications
Western Blot
Synonyms
CEACAM1; Mouse CEACAM1 HEK293 Overexpression Lysate; Mouse CEACAM1/CD66a HEK293 Cell Lysate (WB positive control); carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); Mouse bb-1 Overexpression Lysate; Mouse Bgp Overexpression Lysate; Mouse Bgp1 Overexpression Lysate; Mouse C-CAM Overexpression Lysate; Mouse Cc1 Overexpression Lysate; Mouse CD66a Overexpression Lysate; Mouse Cea-1 Overexpression Lysate; Mouse Cea-7 Overexpression Lysate; Mouse Cea1 Overexpression Lysate; Mouse Cea7 Overexpression Lysate; Mouse Hv-2 Overexpression Lysate; Mouse Hv2 Overexpression Lysate; Mouse mCEA1 Overexpression Lysate; Mouse Mhv-1 Overexpression Lysate; Mouse MHVR Overexpression Lysate; Mouse MHVR1 Overexpression Lysate; Mouse mmCGM1 Overexpression Lysate; Mouse mmCGM1a Overexpression Lysate; Mouse mmCGM2 Overexpression Lysate; CEACAM1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
632aa
Sequence
Met1-Gly428
Applicable Applications for CEACAM1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Ceacam1 (NP_001034274.1) (Met1-Gly428) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CEACAM1 cell lysate
The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
This Mouse CEACAM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CEACAM1 protein from the overexpression lysate was verified.
This Mouse CEACAM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CEACAM1 protein from the overexpression lysate was verified.
Product Categories/Family for CEACAM1 cell lysate
References
Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,271 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 1
NCBI Official Symbol
Ceacam1
NCBI Official Synonym Symbols
Bgp; Cc1; Hv2; Bgp1; Cea1; Cea7; Hv-2; MHVR; bb-1; C-CAM; CD66a; Cea-1; Cea-7; MHVR1; Mhv-1; mCEA1; mmCGM1; mmCGM2; mmCGM1a
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 1; BGP-1; MHV-R; biliary glycoprotein 1; biliary glycoprotein D; hepatitis virus receptor; carcinoembryonic antigen 1; carcinoembryonic antigen 7; murine hepatitis virus receptor; CEA-related cell ad
Protein Family
Similar Products
Product Notes
The CEACAM1 (Catalog #AAA8116701) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 632aa. AAA Biotech's CEACAM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CEACAM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly42 8. It is sometimes possible for the material contained within the vial of "CEACAM1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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