CD4 cell lysate
Human CD4 HEK293 Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Samples are stable for up to twelve months from date of receipt.
This Human CD4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD4 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Uniprot Description
CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft
Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding
Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation
Disease: Okt4 Epitope Deficiency
Research Articles on CD4
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Product Notes
The CD4 cd4 (Catalog #AAA8113819) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 603aa. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Trp39 0. It is sometimes possible for the material contained within the vial of "CD4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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