CD3E & CD3G Heterodimer Cell Lysate | CD3E cell lysate

Human CD3E & CD3G Heterodimer HEK293 Overexpression Lysate

Cat. Num. AAA8118092

• Gene Names: CD3E; T3E; TCRE; IMD18
• Applications: Western Blot
• Synonyms: CD3E & CD3G Heterodimer; Human CD3E & CD3G Heterodimer HEK293 Overexpression Lysate; Human CD3E & CD3G Heterodimer HEK293 Cell Lysate (WB positive control); CD3e molecule; epsilon (CD3-TCR complex); Human CD3E & CD3G Heterodimer Overexpression Lysate; Human IMD18 Overexpression Lysate; Human T3E Overexpression Lysate; Human TCRE Overexpression Lysate; CD3E cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 681 (353+328)aa
• Sequence: Met1-Asp126, Met1-Ser116

Applicable Applications for CD3E cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal DYKDDDDK-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.