CD79 Cell Lysate | CD79A cell lysate
Mouse CD79 HEK293 Overexpression Lysate
Gene Names
Cd79a; Iga; Ly54; mb-1; Ly-54; Igalpha; Ig-alpha
Applications
Western Blot
Synonyms
CD79; Mouse CD79 HEK293 Overexpression Lysate; Mouse CD79A & CD79B Heterodimer HEK293 Cell Lysate (WB positive control); CD79a molecule; immunoglobulin-associated alpha; Mouse Ig-alpha Overexpression Lysate; Mouse Iga Overexpression Lysate; Mouse Igalpha Overexpression Lysate; Mouse Ly-54 Overexpression Lysate; Mouse Ly54 Overexpression Lysate; Mouse mb-1 Overexpression Lysate; CD79A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
264 (120+144)aa
Sequence
MetMet1-Asp158
Applicable Applications for CD79A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse CD79A (NP_031681.2) (Met1-Arg137) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse CD79B (NP_031681.2) (Met1-Asp158) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD79A & CD79B heterodimer was purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CD79A cell lysate
This Mouse CD79 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD79 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,583 Da
NCBI Official Full Name
B-cell antigen receptor complex-associated protein alpha chain
NCBI Official Synonym Full Names
CD79A antigen (immunoglobulin-associated alpha)
NCBI Official Symbol
Cd79a
NCBI Official Synonym Symbols
Iga; Ly54; mb-1; Ly-54; Igalpha; Ig-alpha
NCBI Protein Information
B-cell antigen receptor complex-associated protein alpha chain; Ig alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; immunoglobulin-associated alpha; membrane-bound immunoglobulin-associated protein
UniProt Protein Name
B-cell antigen receptor complex-associated protein alpha chain
Protein Family
UniProt Gene Name
Cd79a
UniProt Synonym Gene Names
Iga; Mb-1
UniProt Entry Name
CD79A_MOUSE
Uniprot Description
Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Cellular Component: multivesicular body; membrane; plasma membrane; integral to membrane; B cell receptor complex; lipid raft; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity
Biological Process: B cell proliferation; B cell receptor signaling pathway; cell surface receptor linked signal transduction; B cell activation; immune system process; B cell differentiation
Research Articles on CD79A
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Product Notes
The CD79A cd79a (Catalog #AAA8118098) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 264 (120+144)aa. AAA Biotech's CD79 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD79A cd79a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MetMet1-As p158. It is sometimes possible for the material contained within the vial of "CD79, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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