CD3E & CD3G Heterodimer Lysate | CD3E&CD3G lysate
Rhesus CD3E & CD3G Heterodimer HEK293 Overexpression Lysate
Applications
Western Blot
Synonyms
CD3E & CD3G Heterodimer; Rhesus CD3E & CD3G Heterodimer HEK293 Overexpression Lysate; Rhesus CD3E & CD3G Heterodimer HEK293 Cell Lysate (WB positive control); CD3e molecule; epsilon (CD3-TCR complex); CD3E&CD3G lysate
Host
HEK293 Cells
Form/Format
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Applicable Applications for CD3E&CD3G lysate
Western Blot (WB)
Application Notes
Western blot (WB): Use at an assay dependent dilution.
Species
Rhesus
Sequence Information
A DNA sequence encoding the rhesus CD3E (XP_014971302.1)(Met1-Asp117) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rhesus CD3G (NP_001253854.1)(Met1-Thr113) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the cynomolgus CD3E/CD3G heterodimer was purified.
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Related Product Information for CD3E&CD3G lysate
Description: This Rhesus CD3E & CD3G Heterodimer overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD3E & CD3G Heterodimer protein from the overexpression lysate was verified.
Background: HEK293 Cell Rhesus CD3E & CD3G Heterodimer transfected/overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Background: HEK293 Cell Rhesus CD3E & CD3G Heterodimer transfected/overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
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Product Notes
The CD3E&CD3G (Catalog #AAA8119764) is a Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD3E & CD3G Heterodimer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot (WB): Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD3E&CD3G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3E & CD3G Heterodimer, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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