PDGFR alpha Active Protein | CD140a active protein

Human PDGFR alpha Recombinant

Cat. Num. AAA553024

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: PDGFR alpha; Human PDGFR alpha Recombinant; Human CD140a Recombinant (PDGF Receptor alpha) ; cd-140 PDGFRA; CD140A; MGC74795; PDGFR2; Rhe-PDGFRA PDGF Receptor alpha; pdgf-r; CD140a active protein

• Host: HEk293 Cells
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CD140a is supplied as a 0.2 um filtered PBS solution, pH7.2.
• Sequence Length: 1089

• Biological Activity: The Activity was measured by the ability to bind human PDGFC-Fc in functional ELISA.
• Domain: Ig-like C2
• Host Note: Optimized DNA sequence encoding Human extracellular domain of human PDGF-R alpha including a C-terminal 6His tag was expressed in HEK293 cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Molecular Weight Note: Recombinant PDGFRa is a monomer protein consisting of 510 amino acid residue subunits, due to glycosylation migrates as an approximately 90-100 kDa protein on SDS-PAGE.
• Preparation and Storage: Recombinant CD140a, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CD140a active protein

The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (PDGFA, PDGFB, PDGFC, and PDGFD. Ligand binding induces receptor dimerization, enabling autophosphorylation of specific tyrosine residues and subsequent recruitment of a variety of signal transduction molecules. PDGFR regulates normal cellular growth and differentiation, and expression of activated PDGFR promotes oncogenic transformation, suggesting that activating mutations or gene rearrangements could play a role in human tumorigenesis. Numerous in vitro and in vivo studies showed that inhibition of PDGFRA signaling disrupts cancer cell survival in the subset of GISTs with activating PDGFRA mutations.

Product Categories/Family for CD140a active protein

Receptors CD

References

• pdgfralpha signaling in the primary cilium regulates nhe1-dependent fibroblast migration via coordinated differential activity of mek1/2-erk1/2-p90rsk and akt signaling pathways .J. Cell Sci., Dec 2012; 10.1242/jcs.116426.
• functional expression of sk channels in murine detrusor pdgfralpha+ cells .J. Physiol., Dec 2012; 10.1113/jphysiol.2012.241505.
• pdgfralpha and cd51 mark human nestin+ sphere-forming mesenchymal stem cells capable of robust hematopoietic stem cell expansion .Blood (ASH Annual Meeting Abstracts), Nov 2012; 120: 505.
• candidate human primary mesenchymal stem/progenitor cells are highly enriched in linneg/cd45neg/cd271pos/pdgfralphaneg bone marrow cells .Blood (ASH Annual Meeting Abstracts), Nov 2012; 120: 3460.
• stromal platelet-derived growth factor receptor alpha (pdgfralpha) provides a therapeutic target independent of tumor cell pdgfralpha expression in lung cancer xenografts .Mol. Cancer Ther., Nov 2012; 11: 2473 - 2482.