CEACAM6 active protein

CEACAM6 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120503

• Gene Names: CEACAM6; NCA; CEAL; CD66c
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CEACAM6; CEACAM6 Protein; Human; Recombinant (His Tag); Human CEACAM6/CD66c Protein (His Tag); CD66c Protein; CEA Protein; CEAL Protein; NCA Protein; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); CEACAM6 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly320

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CEACAM6 (NP_002474.4)(Met1-Gly320) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human CEACAM6-his at 10ug/mL (100uL/well) can bind biotinylated human CEACAM8-his. The EC50 of biotinylated human CEACAM8-his is 0.17ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 ug/mL (100 ul/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 ug/mL.)

Related Product Information for CEACAM6 active protein

Background: Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

References

Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.

NCBI and Uniprot Product Information

• NCBI GI #: 612407822
• NCBI GeneID: 4680
• NCBI Accession #: NP_002474.4
• NCBI GenBank Nucleotide #: NM_002483.6
• UniProt Accession #: P40199; Q13774; Q14920; Q53XP7
• Molecular Weight: 37,195 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 6 preproprotein
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 6
• NCBI Official Symbol: CEACAM6
• NCBI Official Synonym Symbols: NCA; CEAL; CD66c
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 6
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 6
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM6
• UniProt Synonym Gene Names: NCA

NCBI Description

This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19. [provided by RefSeq, Apr 2014]

Uniprot Description

CEACAM6: a glycosylphosphatidylinositol(GPI)-linked glycoprotein implicated in a variety of human cancers. Expressed on epithelial cells of various tissues, especially colon, lung, pancreas, trachea, and bone marrow. Participates in innate immune defense, cell proliferation and differentiation. Expressed at high levels in gastrointestinal tract, pancreatic and lung tumors. CEACAM6 promotes tumor angiogenesis in gastric cancer. May enhance invasiveness and metastatic potential in gastric and pancreatic cancer by promoting EMT via PI3K/AKT signaling. Upregulated by the pro-inflammatory cytokine IL-6. Belongs to the immunoglobulin superfamily/CEA family.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell signaling; leukocyte migration; positive regulation of cell migration; positive regulation of cell proliferation; signal transduction