CD16a / FCGR3A active protein

Recombinant Human CD16a / FCGR3A Protein (176 Val, His & AVI tag)

Cat. Num. AAA2544985

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: CD16a / FCGR3A; Recombinant Human CD16a / FCGR3A Protein (176 Val; His & AVI tag); Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3;CD16;CD16A; CD16a / FCGR3A active protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5%Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Met 1-Gln 208

• Species: Human
• Tag: C-His & AVI
• Bio Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD16a-AVI-His (V158) at 10 ug/ml (100 ul/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.08-0.18 ug/ml.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (400uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for CD16a / FCGR3A active protein

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

NCBI and Uniprot Product Information

• NCBI GI #: 17389688
• NCBI GeneID: 2214
• Molecular Weight: Mol_Mass: 25.6 kDa; AP_Mol_Mass: 46 kDa
• NCBI Official Full Name: FCGR3A protein
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor IIIa
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

NCBI Description

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: external side of plasma membrane; plasma membrane

Biological Process: immune response; regulation of immune response

Disease: Immunodeficiency 20