ACVR2A active protein

ACVR2A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120142

• Gene Names: ACVR2A; ACVR2; ACTRII
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ACVR2A; ACVR2A Protein; Human; Recombinant (His Tag); Human ACVR2/ACTRII/ACVR2A Protein (His Tag); ACTRII Protein; ACVR2 Protein; activin A receptor; type IIA; ACVR2A active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro134

• Species: Human
• Activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.4-3ug/mL in the presence of 10ng/mL recombinant Activin A.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human ACVR2A (NP_001607.1)(Met 1-Pro 134) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.4-3 ug/mL in the presence of 10 ng/mL recombinant Activin A.)

Related Product Information for ACVR2A active protein

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.

References

Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18.Chung H, et al. (2008) Mutation rates of TGFBR2 and ACVR2 coding microsatellites in human cells with defective DNA mismatch repair. PloS one 3(10): e3463.Fitzpatrick E, et al. (2009) Genetic association of the activin A receptor gene (ACVR2A) and pre-eclampsia. Molecular human reproduction 15(3):195-204.Roten LT, et al. (2009) Association between the candidate susceptibility gene ACVR2A on chromosome 2q22 and pre-eclampsia in a large Norwegian population-based study (the HUNT study). EJHG. 17(2): 250-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4501897
• NCBI GeneID: 92
• NCBI Accession #: NP_001607.1
• NCBI GenBank Nucleotide #: NM_001616.4
• UniProt Accession #: P27037; Q53TH4; Q6NWV2; Q92474; B2RAB8; B4DWQ2; D3DP85
• Molecular Weight: 45,713 Da
• NCBI Official Full Name: activin receptor type-2A isoform 1
• NCBI Official Synonym Full Names: activin A receptor, type IIA
• NCBI Official Symbol: ACVR2A
• NCBI Official Synonym Symbols: ACVR2; ACTRII
• NCBI Protein Information: activin receptor type-2A
• UniProt Protein Name: Activin receptor type-2A
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR2A
• UniProt Synonym Gene Names: ACVR2; ACTR-IIA; ACTRIIA
• UniProt Entry Name: AVR2A_HUMAN

NCBI Description

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: EC 2.7.11.30; Protein kinase, TKL; Kinase, protein; Motility/polarity/chemotaxis; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: activin receptor activity; protein self-association; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; PDZ domain binding; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; growth factor binding; activin binding; coreceptor activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: positive regulation of erythrocyte differentiation; embryonic skeletal development; regulation of BMP signaling pathway; activin receptor signaling pathway; Sertoli cell proliferation; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of osteoblast differentiation; positive regulation of activin receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; mesoderm development; spermatogenesis; regulation of nitric-oxide synthase activity; sperm ejaculation; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; penile erection