Acetylcholinesterase Cell Lysate | ACHE cell lysate
Mouse Acetylcholinesterase HEK293 Overexpression Lysate
Gene Names
Ache; mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
Applications
Western Blot
Synonyms
Acetylcholinesterase; Mouse Acetylcholinesterase HEK293 Overexpression Lysate; Mouse Acetylcholinesterase/ACHE HEK293 Cell Lysate (WB positive control); acetylcholinesterase (Yt blood group); Mouse Acre Overexpression Lysate; Mouse Chrne Overexpression Lysate; ACHE cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
594aa
Sequence
Met1-Leu614
Applicable Applications for ACHE cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ACHE cell lysate
Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.
This Mouse Acetylcholinesterase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Acetylcholinesterase protein from the overexpression lysate was verified.
This Mouse Acetylcholinesterase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Acetylcholinesterase protein from the overexpression lysate was verified.
Product Categories/Family for ACHE cell lysate
References
Zhao Y, et al. (2011) Acetylcholinesterase, a key prognostic predictor for hepatocellular carcinoma, suppresses cell growth and induces chemosensitization. Hepatology. 53(2): 493-503.Roepcke CB, et al. (2010) Analysis of phosphorothionate pesticides using a chloroperoxidase pretreatment and acetylcholinesterase biosensor detection. J Agric Food Chem. 58(15): 8748-56.Zaheer-ul-Haq, et al. (2010) Benchmarking docking and scoring protocol for the identification of potential acetylcholinesterase inhibitors. J Mol Graph Model. 28(8): 870-82.Pegan K, et al. (2010) Acetylcholinesterase is involved in apoptosis in the precursors of human muscle regeneration. Chem Biol Interact. 187(1-3): 96-100.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,169 Da
NCBI Official Full Name
acetylcholinesterase
NCBI Official Synonym Full Names
acetylcholinesterase
NCBI Official Symbol
Ache
NCBI Official Synonym Symbols
mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
NCBI Protein Information
acetylcholinesterase
UniProt Protein Name
Acetylcholinesterase
Protein Family
UniProt Gene Name
Ache
UniProt Synonym Gene Names
AChE
Uniprot Description
AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5 G2|5 76.32 cM
Cellular Component: axon; basal lamina; cell junction; cell soma; cell surface; cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; neuron projection; nuclear envelope; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; rough endoplasmic reticulum; synapse
Molecular Function: acetylcholine binding; acetylcholinesterase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity
Biological Process: acetylcholine catabolic process; acetylcholine metabolic process; cell adhesion; choline metabolic process; neurotransmitter catabolic process; neurotransmitter receptor biosynthetic process; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; protein tetramerization; receptor internalization; regulation of receptor recycling; retina development in camera-type eye; synaptogenesis
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Product Notes
The ACHE ache (Catalog #AAA8116684) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 594aa. AAA Biotech's Acetylcholinesterase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ACHE ache for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu61 4. It is sometimes possible for the material contained within the vial of "Acetylcholinesterase, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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