PON1 active protein

Recombinant human PON1 protein

Cat. Num. AAA201237

• Gene Names: PON1; ESA; PON; MVCD5
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: PON1; Recombinant human PON1 protein; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1; paraoxonase 1; ESA; MVCD5; PON; PON1 active protein

• Host: HEK293
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 16-355aa
• Sequence: LFRNHQSSYQ TRLNALREVQ PVELPNCNLV KGIETGSEDL EILPNGLAFI SSGLKYPGIK SFNPNSPGKI LLMDLNEEDP TVLELGITGS KFDVSSFNPH GISTFTDEDN AMYLLVVNHP DAKSTVELFK FQEEEKSLLH LKTIRHKLLP NLNDIVAVGP EHFYGTNDHY FLDPYLQSWE MYLGLAWSYV VYYSPSEVRV VAEGFDFANG INISPDGKYV YIAELLAHKI HVYEKHANWT LTPLKSLDFN TLVDNISVDP ETGDLWVGCH PNGMKIFFYD SENPPASEVL RIQNILTEEP KVTQVYAENG TVLQGSTVAS VYKGKLLIGT VFHKALYCEL
• Sequence Length: 355

• Applicable Applications for PON1 active protein: SDS-PAGE, Enzyme Activity
• Antigen Species: Human
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Predicted Nterm: Leu 16
• Tag Information: His-Tag
• Enzyme Activity: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-Page

(SDS-Page)

Related Product Information for PON1 active protein

PON1, also known as A esterase1, is a member of the paraoxonase family. It is an enzyme that hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). This protein is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis. PON1 shows a variety of atheroprotective properties by metabolizing inflammatory lipid peroxides. It has evolved to be a highly promiscuous enzyme capable of hydrolysing a wide variety of substrates such as lactones, cyclic carbonates, organophosphorus pesticides and nerve gases. Recombinant human PON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for PON1 active protein

Neuroscience

References

Banhela N., et al. (2020) Syst Rev. 9:109.;; Soliman AM., et al. (2020) Eur J Med Chem. 197:112333.

NCBI and Uniprot Product Information

• NCBI GI #: 19923106
• NCBI GeneID: 5444
• NCBI Accession #: NP_000437
• NCBI GenBank Nucleotide #: NP_000437.3
• UniProt Accession #: P27169
• Molecular Weight: 39.0kDa (346aa)
• NCBI Official Full Name: serum paraoxonase/arylesterase 1
• NCBI Official Synonym Full Names: paraoxonase 1
• NCBI Official Symbol: PON1
• NCBI Official Synonym Symbols: ESA; PON; MVCD5
• NCBI Protein Information: serum paraoxonase/arylesterase 1
• UniProt Protein Name: Serum paraoxonase/arylesterase 1
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON1
• UniProt Synonym Gene Names: PON; PON 1
• UniProt Entry Name: PON1_HUMAN

NCBI Description

This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017]

Uniprot Description

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: EC 3.1.8.1; EC 3.1.1.2; Lipid-binding; Secreted, signal peptide; Motility/polarity/chemotaxis; EC 3.1.1.81; Hydrolase; Phosphatase (non-protein); Secreted

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region

Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity

Biological Process: response to external stimulus; response to nutrient levels; dephosphorylation; response to toxin; organophosphate catabolic process; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5