Glial growth factor (GGF2) Active Protein | GGF2 active protein

Human Glial growth factor (GGF2) Recombinant

Cat. Num. AAA553231

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Glial growth factor (GGF2); Human Glial growth factor (GGF2) Recombinant; Human Glial growth factor (GGF2) Recombinant ; egf; ggf2; nrg1; nrg-1; glial; heregulin; hrg; GGF2 active protein

• Host: HEK cells
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant GGF2 was lyophilized from a 0.2 um filtered 0.02M PB, pH 7.0.

• Host Note: Optimized DNA sequence encoding Human Neuregulin-1 isoform 9 GGF2 was expressed in HEK cells.
• Biological Activity: The ED(50) was determined by the dose-dependent proliferation of human MCF-7 cells was found to be <20ng/ml, corresponding to a specific activity of 5x10⁴ Units/mg.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight: Recombinant human GGF2 (Glial growth factor) is a disulfide-linked monomeric protein consisting of 230 amino acid residue subunits, and migrates as an approximately 34 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Product Categories/Family for GGF2 active protein

Cytokines

References

Schwann Cell Isolation and Culture Reveals the Plasticity of These Glia. David E. Weinstein (2017)

NCBI and Uniprot Product Information

• NCBI GI #: 236461509
• NCBI GeneID: 3084
• NCBI Accession #: NP_001153467.1
• NCBI GenBank Nucleotide #: NM_001159995.1
• UniProt Accession #: Q02297; O14667; P98202; Q02298; Q02299; Q07110; Q07111; A5YAK4; A5YAK5; A8K1L2; B7Z4Z3; E9PHH4; Q53F54; Q6ICV5; Q6PK61; Q7RTV8; Q7RTW3; Q7RTW4; Q7RTW5; Q7Z2R7; Q86WJ0
• NCBI Official Full Name: pro-neuregulin-1, membrane-bound isoform isoform HRG-beta1c
• UniProt Protein Name: Pro-neuregulin-1, membrane-bound isoform
• UniProt Gene Name: NRG1
• UniProt Synonym Gene Names: GGF; HGL; HRGA; NDF; SMDF; Pro-NRG1; ARIA; HRG
• UniProt Entry Name: NRG1_HUMAN

NCBI Description

The protein encoded by this gene is a membrane glycoprotein that that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Jun 2014]

Uniprot Description

NRG1: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. Belongs to the neuregulin family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular space; membrane; integral to plasma membrane; axon; apical plasma membrane; cytoplasm; extracellular region; neuromuscular junction; nucleus

Molecular Function: ErbB-2 class receptor binding; protein binding; transmembrane receptor protein tyrosine kinase activator activity; ErbB-3 class receptor binding; growth factor activity; cytokine activity; transcription cofactor activity; protein tyrosine kinase activator activity; receptor tyrosine kinase binding; receptor binding

Biological Process: regulation of protein heterodimerization activity; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; neural crest cell development; wound healing; cellular protein complex disassembly; nerve growth factor receptor signaling pathway; cell morphogenesis; ventricular cardiac muscle cell differentiation; locomotory behavior; positive regulation of striated muscle cell differentiation; cardiac muscle cell differentiation; synaptogenesis; mammary gland development; cell communication; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; neurotransmitter receptor metabolic process; regulation of protein homodimerization activity; neuron fate commitment; MAPKKK cascade; positive regulation of cell growth; peripheral nervous system development; positive regulation of protein kinase B signaling cascade; cell proliferation; embryonic development; glial cell fate commitment; innate immune response; negative regulation of secretion; positive regulation of Ras protein signal transduction; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Schizophrenia 6