SERPINA1/A1AT Active Protein | SERPINA1 active protein

Human CellExp SERPINA1 / A1AT, human recombinant

Cat. Num. AAA844123

• Gene Names: SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
• Applications: SDS-Page
• Purity: >=95% by SDS-PAGE gel
• Synonyms: SERPINA1/A1AT; Human CellExp SERPINA1 / A1AT; human recombinant; SerpinA1; PI; A1A; AAT; PI1; A1AT; MGC9222; PRO2275; MGC23330; alpha1AT; SPAAT; SERPINA1 active protein

• Host: HEK 293 cells(Glu 25 -Lys 418)
• Purity/Purification: >=95% by SDS-PAGE gel
• Form/Format: Lyophilized; Lyophilized from 0.22 um filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
• Sequence Length: 418

• Applicable Applications for SERPINA1 active protein: SDS-PAGE; HPLC
• Molecular Weight Note: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.1 kDa. The predicted N-terminus is Glu 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml.Do not vortex. This solution can be stored at 2-8°C for up to 1 month.For extended storage, it is recommended to store at -20°C
• Endotoxin Level: <1 EU/ug by LAL method
• Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in 100 ul reaction mixture containing 1.25 ng trypsin , 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl , pH 7.5.The IC50 value is approximately <4.5 nM.
• Preparation and Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C and use within 3 months. Avoid repeated freezing and thawing cycles

Testing Data

Related Product Information for SERPINA1 active protein

Background: Serpin A1 also known as Alpha-1-antitrypsin (A1AT), serum trypsin inhibitor, alpha-1 proteinase inhibitor (A1PI), AAT, which belongs to the serpin family. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Serpin A1 / A1AT is inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of SerpinA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Serpin A1 / A1AT protects tissues from enzymes of inflammatory cells, especially neutrophil elastase. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD).

Product Categories/Family for SERPINA1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth

NCBI and Uniprot Product Information

• NCBI GI #: 50363217
• NCBI GeneID: 5265
• NCBI Accession #: NP_000286.3
• NCBI GenBank Nucleotide #: NM_000295.4
• UniProt Accession #: P01009; Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8
• NCBI Official Full Name: alpha-1-antitrypsin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• NCBI Official Symbol: SERPINA1
• NCBI Official Synonym Symbols: PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
• NCBI Protein Information: alpha-1-antitrypsin
• UniProt Protein Name: Alpha-1-antitrypsin
• Protein Family: Alpha-1-antitrypsin
• UniProt Gene Name: SERPINA1
• UniProt Synonym Gene Names: AAT; PI; SPAAT
• UniProt Entry Name: A1AT_HUMAN

NCBI Description

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; glycoprotein binding

Biological Process: platelet activation; response to chromate; platelet degranulation; response to triglyceride; response to cytokine stimulus; response to lead ion; acute-phase response; response to methanol; response to hypoxia; response to lipopolysaccharide; blood coagulation; response to estradiol stimulus

Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive