VEGF 165 Active Protein | VEGFA active protein

Human CellExp VEGF 165, Human Recombinant

Cat. Num. AAA845249

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Purity: > 95% by SDS-PAGE
• Synonyms: VEGF 165; Human CellExp VEGF 165; Human Recombinant; Vascular endothelial growth factor A; MVCD1; VEGF; VEGF165; VPF; MGC70609; Vascular Endothelial Growth Factor A precursor; Vascular Permeability Factor; VEGFA; VEGFA active protein

• Host: HEK 293 cells
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Lyophilized from 0.22 um filtered solution in PBS pH 7.4

• Reconstitution: Centrifuge the vial prior to opening. Reconstitute at 0.2-0.3 mg/mL in distilled water is recommended.
• Biological Activity: EC₅₀ is 600 ng/ml. The activity was determined by the dosedependent stimulation of the proliferation of EA.hy926 human endothelial cells.
• Preparation and Storage: Store at -20°C. After reconstitution, aliquot and store at -80°C. Avoid repeated freezing and thawing cycles.

Testing Data

(Fig A. SDS-PAGE (4-20%) of Human Recombinant VEGF 165: 2 ug of recombinant protein loaded under reducing (Lane R) and non-reducing conditions (Lane NR) and stained with Coomassie Blue. Under reducing and non-reducing conditions the protein migrates as a ~25 kDa (monomer) and ~45 kDa band (homodimer), respectively.Fig B. BIOLOGICAL ACTIVITY: The activity was determined by the dose-dependent stimulation of the proliferation of EA.hy926 human endothelial cells with EC50 600 ng/ml.)

Related Product Information for VEGFA active protein

Vascular Endothelial Growth Factor (VEGF) plays a key role in tumor angiogenesis in many cancers. The VEGF family consists of seven secretory glycoproteins: VEGF-A, VEGF-B, VEGF-C, VEGFD, VEGF-E, VEGF-F and Placental Growth Factor (PlGF). The binding of VEGF and its receptors leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGFA/VEGF165 is a heparin-binding protein, which exists as a disulfide-linked homodimer. It induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Diseases associated with VEGFA/VEGF165 include microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. It is also related to the integrated Breast Cancer Pathway.

Product Categories/Family for VEGFA active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; VEGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A

NCBI and Uniprot Product Information

• NCBI GI #: 76781480
• NCBI GeneID: 7422
• NCBI Accession #: NP_001020537.2
• NCBI GenBank Nucleotide #: NM_001025366.2
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of ~20 kDa (27 -191 aa). Under reducing and non-reducing conditions the protein migrates as a ~25 kDa (monomer) and ~45 kDa band (homodimer) in SDS-PAGE.
• NCBI Official Full Name: vascular endothelial growth factor A isoform a
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.