Thrombomodulin, soluble Active Protein | THBD active protein

Human Thrombomodulin, soluble

Cat. Num. AAA691559

• Gene Names: THBD; TM; THRM; AHUS6; BDCA3; CD141; THPH12
• Reactivity: Human
• Purity: > 98%, by SDS-PAGE and HPLC analysis
• Synonyms: Thrombomodulin; soluble; Human Thrombomodulin; Recombinant Human soluble Thrombomodulin; THBD active protein

• Host: HEK 293 Cells
• Reactivity: Human
• Purity/Purification: > 98%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRS SVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF Q WVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEE QQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA AR GADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCS VE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDL CEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCP Q RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLN QTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALA RHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP A
• Sequence Length: 575

• Endotoxin Level: < 0.1 ng per ug of sThrombomodulin
• Biological Activity: Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.

Related Product Information for THBD active protein

Thrombomodulin (TM, CD141, THBD) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, Thrombin. The Thrombomodulin/Thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of Thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of Thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases. Recombinant soluble Thrombomodulin is a 491 amino acid glycoprotein containing the extracellular domain of Thrombomodulin.

Product Categories/Family for THBD active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4507483
• NCBI GeneID: 7056
• NCBI Accession #: NP_000352.1
• NCBI GenBank Nucleotide #: NM_000361.2
• UniProt Accession #: P07204; Q8IV29; Q9UC32
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: thrombomodulin
• NCBI Official Synonym Full Names: thrombomodulin
• NCBI Official Symbol: THBD
• NCBI Official Synonym Symbols: TM; THRM; AHUS6; BDCA3; CD141; THPH12
• NCBI Protein Information: thrombomodulin; fetomodulin; CD141 antigen
• UniProt Protein Name: Thrombomodulin
• Protein Family: Thrombomodulin
• UniProt Gene Name: THBD
• UniProt Synonym Gene Names: THRM; TM
• UniProt Entry Name: TRBM_HUMAN

NCBI Description

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Endothelial cells are unique in synthesizing thrombomodulin.

Post-translational modification: N-glycosylated. Ref.8The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Involvement in disease: Thrombophilia due to thrombomodulin defect (THPH12) [MIM:614486]: A hemostatic disorder characterized by a tendency to thrombosis.Note: The disease may be caused by mutations affecting the gene represented in this entry. The role of thrombomodulin in thrombosis is controversial. It is likely that genetic or environmental risk factors in addition to THBD variation are involved in the pathogenesis of venous thrombosis. Ref.15 Ref.16 Ref.20Hemolytic uremic syndrome atypical 6 (AHUS6) [MIM:612926]: An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Other genes may play a role in modifying the phenotype. Ref.21 Ref.22

Sequence similarities: Contains 1 C-type lectin domain.Contains 6 EGF-like domains.