SDS-PAGE (SDS)
(SDS-PAGE under reducing conditions and visualized by Coomassie blue staining showing a band at 17 KDa and one at 18 KDa corresponding to the glycosylated form. Western Blot: IL-2 as antigen (with mouse monoclonal Antibody B-G5 #MBS335411) chemiluminescence detection)
Interleukin-2 (IL-2) Active Protein | IL-2 active protein
Recombinant Human Interleukin-2 (IL-2)
Purity
Purity: >95% by SDS PAGE gelPurification: Affinity chromatography followed by Ion exchange chromatography
Synonyms
Interleukin-2 (IL-2); Recombinant Human Interleukin-2 (IL-2); IL-2 active protein
Host
HEK 293 cells
Purity/Purification
Purity: >95% by SDS PAGE gel
Purification: Affinity chromatography followed by Ion exchange chromatography
Purification: Affinity chromatography followed by Ion exchange chromatography
Form/Format
Lyophilized from PBS pH 7.4 (NaCl 155mM, Na2HPO4 8mM & KH2PO4, 1.8mM, Trehalose)
Concentration
10ug / vial – 125 ug/ml (715 H02.010-CF)
25 ug/ vial – 125 ug/ml (715 H02.025-CF) (varies by lot)
25 ug/ vial – 125 ug/ml (715 H02.025-CF) (varies by lot)
Application Notes
SDS Page: 17 KDa & 18 KDa, reducing conditions ; second band results of glycosylation (confirmed by mass spectrometry)
Source Note
Human IL-2 produced in HEK 293 cells
Tag
His Tag C-Terminus
Extinction Coefficient
10 095 M -1 .cm -1, Abs 0.1%(=1 g/l) 0.617 assuming all pairs residues from cysteines
Reconstitution
Centrifuge the vial prior opening It is recommended to reconstitute in sterile water at 100 ug/ml
Endotoxins
Negative on cell based assay
Activity
Measured in a cell based assay on stimulated Kit 225 cells (EC50= 0.2 ng/ml)
Preparation and Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Stable one week at 2 to 8°C after reconstitution under sterile conditions.
Stable 12 months -20°C to -80°C after reconstitution under sterile conditions.
Stable one week at 2 to 8°C after reconstitution under sterile conditions.
Stable 12 months -20°C to -80°C after reconstitution under sterile conditions.
SDS-PAGE (SDS)
(SDS-PAGE under reducing conditions and visualized by Coomassie blue staining showing a band at 17 KDa and one at 18 KDa corresponding to the glycosylated form. Western Blot: IL-2 as antigen (with mouse monoclonal Antibody B-G5 #MBS335411) chemiluminescence detection)
SDS-PAGE (SDS)
(SDS-PAGE under reducing conditions and visualized by Coomassie blue staining showing a band at 17 KDa and one at 18 KDa corresponding to the glycosylated form. Western Blot: IL-2 as antigen (with mouse monoclonal Antibody B-G5 #MBS335411) chemiluminescence detection)
Testing Data
(Biological Activity measured in a cell proliferation assay using Kit 225 cells The EC50 for this effect is 0.2 ng/ml)
Testing Data
(Biological Activity measured in a cell proliferation assay using Kit 225 cells The EC50 for this effect is 0.2 ng/ml)
Related Product Information for IL-2 active protein
IL-2 is a powerful immunoregulatory lymphokine produced by T-Cells in response to antigenic or mitogenic activation (1). IL-2 stimulates growth and differentiation of B-Cells, most T-cells, NK cells, monocytes and macrophages (2,3,4). Mature IL-2 is a 15.4kDa globular glycoprotein containing 133 amino acid residues including one intrachain disulfide bond between residues 58 and 105 (5). Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation (6), IL-2 modulates the expression of interferon-y(7) and major histocompatibility antigens. Alterations in the ability of T-cell to synthesize IL-2 have been observed in physiologic and pathologic states. Currently, IL-2 is used to enhance the immune system of patients for the treatment of cancer and infectious disease.
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Product Notes
The IL-2 (Catalog #AAA335850) is an Active Protein produced from HEK 293 cells and is intended for research purposes only. The product is available for immediate purchase. SDS Page: 17 KDa & 18 KDa, reducing conditions ; second band results of glycosylation (confirmed by mass spectrometry). Researchers should empirically determine the suitability of the IL-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin-2 (IL-2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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