HDAC8 / Histone Deacetylase 8 Recombinant Protein | HDAC8 recombinant protein

Human HDAC8 / Histone Deacetylase 8 Recombinant Protein

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; KDAC8; MRXS6; HDACL1
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: HDAC8 / Histone Deacetylase 8; Human HDAC8 / Histone Deacetylase 8 Recombinant Protein; Histone deacetylase 8; HD8; HDACL1; CDA07; HDAC8 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 377

• Applicable Applications for HDAC8 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv(16)(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate in the conversion of RUNX1 into a constitutive transcriptional repressor. Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin).
• Entry Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 29839394
• NCBI GeneID: 55869
• UniProt Accession #: Q9BY41
• NCBI Official Full Name: Histone deacetylase 8
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; KDAC8; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8
• UniProt Protein Name: Histone deacetylase 8
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome