Typical Testing Data/Standard Curve (for reference only)
Human Histone Deacetylase 6 ELISA Kit | HDAC6 elisa kit
Human Histone Deacetylase 6 ELISA Kit
Gene Names
Hdac6; Hd6; Sfc6; Hdac5; mHDA2
Reactivity
Human
Synonyms
Histone Deacetylase 6; Human Histone Deacetylase 6 ELISA Kit; HDAC6 elisa kit
Reactivity
Human
Sequence Length
1149
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 umol/L.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HDAC6 elisa kit
Intended Uses: This HCY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HCY. This ELISA kit for research use only!
Principle of the Assay: HCY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HCY antibody and an HCY-HRP conjugate. The assay sample and buffer are incubated together with HCY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCY concentration since HCY from samples and HCY-HRP conjugate compete for the anti-HCY antibody binding site. Since the number of sites is limited, as more sites are occupied by HCY from the sample, fewer sites are left to bind HCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCY concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HCY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HCY antibody and an HCY-HRP conjugate. The assay sample and buffer are incubated together with HCY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCY concentration since HCY from samples and HCY-HRP conjugate compete for the anti-HCY antibody binding site. Since the number of sites is limited, as more sites are occupied by HCY from the sample, fewer sites are left to bind HCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCY concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125,787 Da
NCBI Official Full Name
histone deacetylase 6
NCBI Official Synonym Full Names
histone deacetylase 6
NCBI Official Symbol
Hdac6
NCBI Official Synonym Symbols
Hd6; Sfc6; Hdac5; mHDA2
NCBI Protein Information
histone deacetylase 6
UniProt Protein Name
Histone deacetylase 6
Protein Family
UniProt Gene Name
Hdac6
UniProt Synonym Gene Names
HD6
Uniprot Description
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Research Articles on HDAC6
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Product Notes
The Human HDAC6 hdac6 (Catalog #AAA7254230) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7254230 ELISA Kit recognizes Human HDAC6. It is sometimes possible for the material contained within the vial of "Histone Deacetylase 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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