Monkey Histone Deacetylase 2 ELISA Kit | HDAC2 elisa kit

Monkey Histone Deacetylase 2 ELISA Kit

Cat. Num. AAA755683

• Gene Names: HDAC2; HD2; RPD3; YAF1
• Reactivity: Monkey
• Synonyms: Histone Deacetylase 2; Monkey Histone Deacetylase 2 ELISA Kit; HDAC2 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC2 elisa kit

Principle of the assay: HDAC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HDAC2 antibody and an HDAC2-HRP conjugate. The assay sample and buffer are incubated together with HDAC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HDAC2 concentration since HDAC2 from samples and HDAC2-HRP conjugate compete for the anti-HDAC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HDAC2 from the sample, fewer sites are left to bind HDAC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDAC2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HDAC2 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 293336691
• NCBI GeneID: 3066
• NCBI Accession #: NP_001518.3
• NCBI GenBank Nucleotide #: NM_001527.3
• Molecular Weight: 55,364 Da
• NCBI Official Full Name: histone deacetylase 2
• NCBI Official Synonym Full Names: histone deacetylase 2
• NCBI Official Symbol: HDAC2
• NCBI Official Synonym Symbols: HD2; RPD3; YAF1
• NCBI Protein Information: histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3
• UniProt Protein Name: Histone deacetylase 2
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC2
• UniProt Synonym Gene Names: HD2
• UniProt Entry Name: HDAC2_HUMAN

NCBI Description

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; Transcription, coactivator/corepressor; EC 3.5.1.98; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; cytoplasm; nuclear chromatin; replication fork; NuRD complex; nucleus

Molecular Function: deacetylase activity; nucleosomal DNA binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor activity

Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of gene expression, epigenetic; negative regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; negative regulation of MHC class II biosynthetic process; maintenance of chromatin silencing; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis