Human Histone Deacetylase 2 ELISA Kit | HDAC2 elisa kit

Human Histone Deacetylase 2 ELISA Kit

Cat. Num. AAA762081

• Gene Names: HDAC2; HD2; RPD3; YAF1
• Reactivity: Human
• Synonyms: Histone Deacetylase 2; Human Histone Deacetylase 2 ELISA Kit; HDAC2/YAF1/HD2/histone deacetylase 2/RPD3/transcriptional regulator homolog RPD3/YAF1/YY1-associated factor 1; HDAC2 elisa kit

• Reactivity: Human
• Sequence Length: 488

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HDAC2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HDAC2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HDAC2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HDAC2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HDAC2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 293336691
• NCBI GeneID: 3066
• NCBI Accession #: NP_001518.3
• NCBI GenBank Nucleotide #: NM_001527.3
• Molecular Weight: 51,998 Da
• NCBI Official Full Name: histone deacetylase 2
• NCBI Official Synonym Full Names: histone deacetylase 2
• NCBI Official Symbol: HDAC2
• NCBI Official Synonym Symbols: HD2; RPD3; YAF1
• NCBI Protein Information: histone deacetylase 2
• UniProt Protein Name: Histone deacetylase 2
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC2
• UniProt Synonym Gene Names: HD2
• UniProt Entry Name: HDAC2_HUMAN

NCBI Description

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; Nuclear receptor co-regulator; EC 3.5.1.98; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; ESC/E(Z) complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex

Molecular Function: chromatin binding; deacetylase activity; enzyme binding; heat shock protein binding; histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; sequence-specific DNA binding; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; behavioral response to ethanol; blood coagulation; chromatin remodeling; circadian regulation of gene expression; dendrite development; embryonic digit morphogenesis; epidermal cell differentiation; establishment and/or maintenance of chromatin architecture; gene expression; histone deacetylation; maintenance of chromatin silencing; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of DNA binding; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of interleukin-1 production; positive regulation of oligodendrocyte differentiation; positive regulation of proteolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of gene expression, epigenetic; response to amphetamine; response to caffeine; response to cocaine; response to drug; response to hyperoxia; response to lipopolysaccharide; response to nicotine; transcription, DNA-dependent