Bovine Hemoglobin, Mu ELISA Kit | HBmu elisa kit

Bovine Hemoglobin, Mu ELISA Kit

Cat. Num. AAA743956

• Gene Names: HBM; HBK; HBAP2
• Reactivity: Bovine
• Synonyms: Hemoglobin; Mu; Bovine Hemoglobin; Mu ELISA Kit; HBmu elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This HBu ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HBu. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This HBu ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HBu. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HBmu elisa kit

Principle of the Assay: HBu ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBu antibody and an HBu-HRP conjugate. The assay sample and buffer are incubated together with HBu-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBu concentration since HBu from samples and HBu-HRP conjugate compete for the anti-HBu antibody bindingsite. Since the number of sites is limited, as more sites are occupied by from the sample, fewer sites are left to bind HBu-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standard. The HBu concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HBmu elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 51510893
• NCBI GeneID: 3042
• NCBI Accession #: NP_001003938.1
• NCBI GenBank Nucleotide #: NM_001003938.3
• Molecular Weight: 15,618 Da
• NCBI Official Full Name: hemoglobin subunit mu
• NCBI Official Synonym Full Names: hemoglobin, mu
• NCBI Official Symbol: HBM
• NCBI Official Synonym Symbols: HBK; HBAP2
• NCBI Protein Information: hemoglobin subunit mu; mu-globin; hemoglobin mu chain; alpha globin pseudogene 2; hemoglobin, alpha pseudogene 2
• UniProt Protein Name: Hemoglobin subunit mu
• UniProt Gene Name: HBM
• UniProt Synonym Gene Names: HBAP2
• UniProt Entry Name: HBM_HUMAN

NCBI Description

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. This gene has an ORF encoding a 141 aa polypeptide which is similar to the delta globins found in reptiles and birds. This locus was originally described as a pseudogene; however, it is currently thought to be a protein-coding gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HBM: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. This gene has an ORF encoding a 141 aa polypeptide which is similar to the delta globins found in reptiles and birds. This locus was originally described as a pseudogene; however, it is currently thought to be a protein-coding gene. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: hemoglobin complex

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport