Human heparin-binding EGF-like growth factor ELISA Kit | HBEGF elisa kit

Human heparin-binding epidermal growth factor-like growth factor, HB-EGF ELISA Kit

Cat. Num. AAA704504

• Gene Names: HBEGF; DTR; DTS; DTSF; HEGFL
• Reactivity: Human
• Synonyms: heparin-binding EGF-like growth factor; Human heparin-binding epidermal growth factor-like growth factor; HB-EGF ELISA Kit; DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor) ; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ; heparin-binding epide; HBEGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human HBEGF. No significant cross-reactivity or interference between Human HBEGF and analogues was observed.
• Sequence Length: 208

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HBEGF elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HB-EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HB-EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HB-EGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HB-EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503413
• NCBI GeneID: 1839
• NCBI Accession #: NP_001936.1
• NCBI GenBank Nucleotide #: NM_001945.2
• UniProt Accession #: Q99075; B2R821
• Molecular Weight: 23,067 Da
• NCBI Official Full Name: proheparin-binding EGF-like growth factor
• NCBI Official Synonym Full Names: heparin-binding EGF-like growth factor
• NCBI Official Symbol: HBEGF
• NCBI Official Synonym Symbols: DTR; DTS; DTSF; HEGFL
• NCBI Protein Information: proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
• UniProt Protein Name: Proheparin-binding EGF-like growth factor
• Protein Family: Proheparin-binding EGF-like growth factor
• UniProt Gene Name: HBEGF
• UniProt Synonym Gene Names: DTR; DTS; HEGFL; HB-EGF; HBEGF; DT-R
• UniProt Entry Name: HBEGF_HUMAN

Uniprot Description

Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.