HARS recombinant protein

HARS, 1-509aa, Human, His tag, E Coli

Cat. Num. AAA203575

• Gene Names: HARS; HRS; CMT2W; USH3B
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HARS; 1-509aa; Human; His tag; E Coli; Histidyl-tRNA synthetase cytoplasmic; FLJ20491; HRS; HisRS; Histidine--tRNA ligase; Histidyl-tRNA synthetase; cytoplasmic; HARS recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC
• Sequence Length: 509

• Applicable Applications for HARS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HARS recombinant protein

HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for HARS recombinant protein

Enzymes & Proteases

References

Raben N., et al. (1992) Nucleic Acids Res. 20 (5):1075-81. ; O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294 (3):609-14.;

NCBI and Uniprot Product Information

• NCBI GI #: 6996014
• NCBI GeneID: 3035
• NCBI Accession #: NP_002100
• NCBI GenBank Nucleotide #: NM_002109.5
• UniProt Accession #: P12081; B4DHQ1; B4DY73; D6REN6; J3KNE5
• Molecular Weight: 59.4 kDa (532aa), confirmed by MALDI-TOF
• NCBI Official Full Name: histidine--tRNA ligase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: histidyl-tRNA synthetase
• NCBI Official Symbol: HARS
• NCBI Official Synonym Symbols: HRS; CMT2W; USH3B
• NCBI Protein Information: histidine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Histidine--tRNA ligase, cytoplasmic
• Protein Family: Histidine--tRNA ligase
• UniProt Gene Name: HARS
• UniProt Synonym Gene Names: HRS; HisRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Cytoplasmic histidine--tRNA ligase (Probable). Plays a role in axon guidance.