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3-isopropylmalate dehydratase large subunit 2 (leuC2) Recombinant Protein | leuC2 recombinant protein

Recombinant Methanothermobacter thermautotrophicus 3-isopropylmalate dehydratase large subunit 2 (leuC2)

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
3-isopropylmalate dehydratase large subunit 2 (leuC2); Recombinant Methanothermobacter thermautotrophicus 3-isopropylmalate dehydratase large subunit 2 (leuC2); 3-isopropylmalate dehydratase large subunit 2; EC=4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2; leuC2 recombinant protein
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For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-428aa; full length protein
Sequence
MVKMNMTEKI LAEAAGLREV TPGEIIEARV DLAMTHDGTS PPTIRTFRDI ASRGGPARVWDPERIVMVFD HNVPANTIGA AEFQRVTREF AREQGIVNIF QNAAGICHQV LPERGFVRPGMVIVGADSHT CTYGAFGAFA TGMGATDMAM VFATGKTWFM VPEAMRIEVT GEPEGHVYAKDVILHIIGEI GVDGATYRSV EFTGDTIESM DVSGRMTICN MAVEMGAKNG IMEPNRQTLDYVRARTGREF RVYSSDEDSQ YLEDHHFDVS DLEPQVACPD DVDNVYPVHR VEGTHIDEAFLGSCTNGRYE DLKIAAEVIG DRRVHEDVRF IVSPASREIY LKALEDGIIE TFIRAGAIVCNPGCGPCLGA HMGVLAPGEV SIATTNRNFR GRMGDPASSV YLANPAVVAE SAIEGVISAPQQEAGNGC
Sequence Length
428
Species
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,448 Da
NCBI Official Full Name
3-isopropylmalate dehydratase large subunit
NCBI Official Symbol
MTH1631
NCBI Protein Information
3-isopropylmalate dehydratase large subunit
UniProt Protein Name
Probable methanogen homoaconitase large subunit
UniProt Gene Name
hacA
UniProt Synonym Gene Names
HACN
UniProt Entry Name
HACA_METTH

Uniprot Description

Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate. All these reactions are part of the biosynthesis pathway of coenzyme B ().

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Product Notes

The leuC2 haca (Catalog #AAA1236878) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-428aa; full length protein. The amino acid sequence is listed below: MVKMNMTEKI LAEAAGLREV TPGEIIEARV DLAMTHDGTS PPTIRTFRDI ASRGGPARVW DPERIVMVFD HNVPANTIGA AEFQRVTREF AREQGIVNIF QNAAGICHQV LPERGFVRPG MVIVGADSHT CTYGAFGAFA TGMGATDMAM VFATGKTWFM VPEAMRIEVT GEPEGHVYAK DVILHIIGEI GVDGATYRSV EFTGDTIESM DVSGRMTICN MAVEMGAKNG IMEPNRQTLD YVRARTGREF RVYSSDEDSQ YLEDHHFDVS DLEPQVACPD DVDNVYPVHR VEGTHIDEAF LGSCTNGRYE DLKIAAEVIG DRRVHEDVRF IVSPASREIY LKALEDGIIE TFIRAGAIVC NPGCGPCLGA HMGVLAPGEV SIATTNRNFR GRMGDPASSV YLANPAVVAE SAIEGVISAP QQEAGNGC. It is sometimes possible for the material contained within the vial of "3-isopropylmalate dehydratase large subunit 2 (leuC2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.
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