H1N1/HA1 recombinant protein

H1N1/HA1, 18-344aa, Influenza A-H1N1 (human), His tag, Baculovirus

Cat. Num. AAA204133

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: H1N1/HA1; 18-344aa; Influenza A-H1N1 (human); His tag; Baculovirus; hemagglutinin; Influenza A virus (A/New York/3571/2009 H1N1) haemagglutinin; H1N1/HA1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: ADLMDTLCIG YHANNSTDTV DTVLEKNVTV THSVNLLEDK HNGKLCKLRG VAPLHLGKCN IAGWILGNPE CESLSTASSW SYIVETSSSD NGTCYPGDFI DYEELREQLS SVSSFERFEI FPKTSSWPNH DSNKGVTAAC PHAGAKSFYK NLIWLVKKGN SYPKLSKSYI NDKGKEVLVL WGIHHPSTSA DQQSLYQNAD AYVFVGSSRY SKKFKPEIAI RPKVRDQEGR MNYYWTLVEP GDKITFEATG NLVVPRYAFA MERNAGSGII ISDTPVHDCN TTCQTPKGAI NTSLPFQNIH PITIGKCPKY VKSTKLRLAT GLRNVPSIQS RSRHHHHHH
• Sequence Length: 566

• Applicable Applications for H1N1/HA1 recombinant protein: SDS-PAGE
• Antigen Species: Influenza A-H1N1
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for H1N1/HA1 recombinant protein

HA1 (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/ New York/3571/2009 (H1N1)) HA1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

References

Russell RJ, et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105 (46): 17736-41.; White JM, et al. (1997) Structural Biology of Viruses. Oxford university Press. pp. 80-104.

NCBI and Uniprot Product Information

• NCBI GI #: 256595204
• Molecular Weight: 37.8kDa (339aa)
• NCBI Official Full Name: hemagglutinin
• UniProt Protein Name: Hemagglutinin
• UniProt Gene Name: HA

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.