Glycogenin-1 Recombinant Protein | GYG1 recombinant protein

Recombinant Human Glycogenin-1

Cat. Num. AAA143849

• Gene Names: GYG1; GYG; GSD15
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Glycogenin-1; Recombinant Human Glycogenin-1; GYG1 Human; Glycogenin-1 Human Recombinant; GYG1; GYG; GYG1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The GYG1 solution contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMTDQAFVT LTTNDAYAKG ALVLGSSLKQ HRTTRRLVVL ATPQVSDSMR KVLETVFDEV IMVDVLDSGD SAHLTLMKRP ELGVTLTKLH CWSLTQYSKC VFMDADTLVL ANIDDLFDRE ELSAAPDPGW PDCFNSGVFV YQPSVETYNQ LLHLASEQGS FDGGDQGILN TFFSSWATTD IRKHLPFIYN LSSISIYSYL PAFKVFGASA KVVHFLGRVK PWNYTYDPKT KSVKSEAHDP NMTHPEFLIL WWNIFTTNVL PLLQQFGLVK DTCSYVNVED VSGAISHLSL GEIPAMAQPF VSSEERKERW EQGQADYMGA DSFDNIKRKL DTYLQ
• Sequence Length: 333

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for GYG1 recombinant protein

Description: GYG1 Human Recombinant fused with a 32 amino acid His-T7 tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 365 amino acids (1-333 a.a.) and having a molecular mass of 41.2kDa.The GYG1 is purified by proprietary chromatographic techniques.

Introduction: Glycogenin-1 (GYG1) is an enzyme involved in glycogen biosynthesis. GYG1 is the chief enzyme involved in glycogen polymerisation. Glycogenin-1 is vital for the function of self-glucosylates, using an inter-subunit mechanism, to form an oligosaccharide primer which acts as substrate for glycogen synthase. In addition, GYG1 has a role in regulating glycogen metabolism and the achievement of maximal glycogen levels in skeletal muscle. GYG1 mRNA and protein content and activity increase in the muscle during recovery from prolonged and exhaustive exercise. GYG1 is inactivated with glycogen catabolism which concurs with an increase in glycogenin gene expression as exercise and glycogenolysis advance. Glycogenin will remain covalently attached to the reducing end of the glycogen molecule.

Product Categories/Family for GYG1 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 296040505
• NCBI GeneID: 2992
• NCBI Accession #: NP_001171649.1
• NCBI GenBank Nucleotide #: NM_001184720.1
• UniProt Accession #: P46976; Q6FHZ1; Q9UNV0; D3DNH0; D3DNH1; D3DNH2
• Molecular Weight: 31,467 Da
• NCBI Official Full Name: glycogenin-1 isoform 2
• NCBI Official Synonym Full Names: glycogenin 1
• NCBI Official Symbol: GYG1
• NCBI Official Synonym Symbols: GYG; GSD15
• NCBI Protein Information: glycogenin-1; GN-1; glycogenin glucosyltransferase
• UniProt Protein Name: Glycogenin-1
• Protein Family: Glycogenin
• UniProt Gene Name: GYG1
• UniProt Synonym Gene Names: GYG; GN-1; GN1
• UniProt Entry Name: GLYG_HUMAN

NCBI Description

This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

glycogenin: Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Defects in GYG1 are the cause of glycogen storage disease type 15 (GSD15). It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation. Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.186; Transferase

Chromosomal Location of Human Ortholog: 3q24-q25.1

Cellular Component: membrane; cytosol

Molecular Function: protein binding; metal ion binding; glycogenin glucosyltransferase activity; glucose binding

Biological Process: glycogen biosynthetic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis