Guinea Pig PON3 ELISA Kit | PON3 elisa kit
Guinea pig PON3 (Paraoxonase 3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PON3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PON3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PON3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PON3. You can calculate the concentration of PON3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
PON3: Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents. Belongs to the paraoxonase family.
Protein type: EC 3.1.1.81; EC 3.1.1.2; Secreted, signal peptide; EC 3.1.8.1; Hydrolase; Secreted
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: extracellular space; intracellular membrane-bound organelle
Molecular Function: protein homodimerization activity; 3,4-dihydrocoumarin hydrolase activity; arylesterase activity; dihydrocoumarin hydrolase activity; metal ion binding; aryldialkylphosphatase activity
Biological Process: response to external stimulus; dephosphorylation; coumarin catabolic process; carboxylic acid catabolic process; aromatic compound catabolic process; phenylacetate catabolic process
