Guinea Pig FBN3 ELISA Kit | FBN3 elisa kit

Guinea pig FBN3 (Fibrillin 3) ELISA Kit

Cat. Num. AAA2504511

• Reactivity: Guinea Pig
• Synonyms: FBN3; Guinea pig FBN3 (Fibrillin 3) ELISA Kit; FBN3 elisa kit

• Reactivity: Guinea Pig
• Specificity: This kit recognizes natural and recombinant Guinea pig FBN3. No significant cross-reactivity or interference between Guinea pig FBN3 and analogues was observed.
• Sequence Length: 2809

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBN3 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig FBN3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FBN3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FBN3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FBN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FBN3. You can calculate the concentration of FBN3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56237021
• NCBI GeneID: 84467
• NCBI Accession #: NP_115823.3
• NCBI GenBank Nucleotide #: NM_032447.3
• UniProt Accession #: Q75N90; Q75N91; Q75N92; Q75N93; Q86SJ5; Q96JP8
• Molecular Weight: 300,356 Da
• NCBI Official Full Name: fibrillin-3
• NCBI Official Synonym Full Names: fibrillin 3
• NCBI Official Symbol: FBN3
• NCBI Protein Information: fibrillin-3
• UniProt Protein Name: Fibrillin-3
• Protein Family: Fibrillin
• UniProt Gene Name: FBN3
• UniProt Synonym Gene Names: KIAA1776
• UniProt Entry Name: FBN3_HUMAN

NCBI Description

This gene encodes a protein that belongs to the fibrillin gene family. Fibrillins are extracellular matrix molecules that assemble into microfibrils in many connective tissues. This gene is most highly expressed in fetal tissues and its protein product is localized to extracellular microfibrils of developing skeletal elements, skin, lung, kidney, and skeletal muscle. This gene is potentially involved in Weill-Marchesani syndrome. While several transcript variants may exist for this gene, their full-length natures have not been described to date. [provided by RefSeq, Jul 2008]

Uniprot Description

FBN3: Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin- containing microfibrils provide long-term force bearing structural support. Belongs to the fibrillin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; calcium ion binding