TFII I Blocking Peptide | GTF2I blocking peptide

TFII I Blocking Peptide

Cat. Num. AAA9622033

• Gene Names: GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: TFII I; TFII I Blocking Peptide; BAP 135; BAP-135; BAP135; Bruton tyrosine kinase associated protein 135; Bruton tyrosine kinase-associated protein 135; BTK associated protein 135; BTK associated protein 135kD; BTK associated protein; BTK-associated protein 135; BTKAP 1; BTKAP1; DIWS; FLJ38776; FLJ56355; General transcription factor II i; General transcription factor II-I; General transcription factor IIi; GTF 2I; Gtf2i; GTF2I_HUMAN; GTFII I; GTFII-I; IB 291; IB291; SPIN; SRF Phox 1 interacting protein; SRF Phox1 interacting protein; SRF-Phox1-interacting protein; TFII-I; Transcription factor II I; WBS; WBSCR 6; WBSCR6; Williams Beuren syndrome chromosome region 6; Williams Beuren syndrome chromosome region 6 protein; Williams-Beuren syndrome chromosomal region 6 protein; GTF2I blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for GTF2I blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GTF2I blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 254692934
• NCBI GeneID: 2969
• NCBI Accession #: NP_001157108.1
• NCBI GenBank Nucleotide #: NP_001157108.1
• UniProt Accession #: P78347; O14743; O15359; O43546; O43588; O43589; Q75M85; Q75M86; Q75M87; Q75M88; Q9BSZ4
• Molecular Weight: 112,416 Da
• NCBI Official Full Name: general transcription factor II-I isoform 5
• NCBI Official Synonym Full Names: general transcription factor IIi
• NCBI Official Symbol: GTF2I
• NCBI Official Synonym Symbols: WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
• NCBI Protein Information: general transcription factor II-I; BTK-associated protein 135; BTK-associated protein, 135kD; SRF-Phox1-interacting protein; Williams-Beuren syndrome chromosome region 6; Bruton tyrosine kinase-associated protein 135; williams-Beuren syndrome chromosomal
• UniProt Protein Name: General transcription factor II-I
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2I
• UniProt Synonym Gene Names: BAP135; WBSCR6; GTFII-I; TFII-I; BAP-135; BTK-associated protein 135; SPIN
• UniProt Entry Name: GTF2I_HUMAN

NCBI Description

This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7, 13 and 21. [provided by RefSeq, Jul 2009]

Uniprot Description

GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; cell projection; membrane; cell soma; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; transition between slow and fast fiber; reduction of cytosolic calcium ion concentration; signal transduction

Disease: Williams-beuren Syndrome