Bovine General Transcription Factor IIF, Polypeptide 1 ELISA Kit | GTF2F1 elisa kit

Bovine General Transcription Factor IIF, Polypeptide 1 ELISA Kit

Cat. Num. AAA752935

• Gene Names: GTF2F1; BTF4; RAP74; TF2F1; TFIIF
• Reactivity: Bovine
• Synonyms: General Transcription Factor IIF; Polypeptide 1; Bovine General Transcription Factor IIF; Polypeptide 1 ELISA Kit; GTF2F1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GTF2F1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GTF2F1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GTF2F1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GTF2F1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GTF2F1 elisa kit

Principle of the Assay: GTF2F1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GTF2F1 antibody and an GTF2F1-HRP conjugate. The assay sample and buffer are incubated together with GTF2F1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GTF2F1 concentration since GTF2F1 from samples and GTF2F1-HRP conjugate compete for the anti-GTF2F1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GTF2F1 from the sample, fewer sites are left to bind GTF2F1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GTF2F1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GTF2F1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 48145713
• NCBI GeneID: 2962
• UniProt Accession #: Q6IBK5; P35269
• Molecular Weight: 58,226 Da
• NCBI Official Full Name: GTF2F1
• NCBI Official Synonym Full Names: general transcription factor IIF, polypeptide 1, 74kDa
• NCBI Official Symbol: GTF2F1
• NCBI Official Synonym Symbols: BTF4; RAP74; TF2F1; TFIIF
• NCBI Protein Information: general transcription factor IIF subunit 1; TFIIF-alpha; transcription initiation factor RAP74; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha
• UniProt Protein Name: GTF2F1 protein
• Protein Family: General transcription factor
• UniProt Gene Name: GTF2F1
• UniProt Entry Name: Q6IBK5_HUMAN

Uniprot Description

GTF2F1: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family.

Protein type: Kinase, protein; EC 2.7.11.1; Transcription initiation complex; ATYPICAL group; GTF2F1 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription factor TFIIF complex; nucleus; cell junction

Molecular Function: protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; transcription factor binding; catalytic activity

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation from RNA polymerase II promoter; RNA splicing; response to virus; RNA elongation from RNA polymerase II promoter; gene expression